• USA Home
  • A2024 - Apramycin sulfate salt

EMAIL THIS PAGE TO A FRIEND

A2024 Sigma

Apramycin sulfate salt

~95% (TLC)

Synonym: Nebramycin II

Purchase

Properties

Related Categories A - K, Aminoglycosides, Antibacterial, Antibiotics, Antibiotics A to Z,
assay   ~95% (TLC)
solubility   H2O: soluble25 mg/mL
antibiotic activity spectrum   Gram-negative bacteria
storage temp.   2-8°C

Description

Application

Apramycin is used to study antibiotic resistance 1,2 as well as protein synthesis translocation-step inhibition in bacteria and prokaryotes.

Biochem/physiol Actions

Apramycin inhibits protein synthesis by blocking translocation. It is also able to bind to the eukaryotic decoding site.3 At low concentrations it inhibits elongation and induces misreading of mRNA during protein synthesis.4

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.



Customers Also Viewed

Paromomycin sulfate salt

≥98% (TLC)

Paromomycin sulfate salt

powder, BioReagent, suitable for cell culture, ≥98%

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 3264 8 / PGIII
WGK Germany 
3

Protocols & Articles

Articles

Aminoglycoside Analysis in Honey Using Molecularly Imprinted Polymer Cleanup and LC/MS/MS Detection

Introduction Streptomycin and related compounds belong to a well-known class of antibiotics, the aminoglycosides. They are widely used to control bacterial and fungal diseases of fruit trees, seeds, ...
Keywords: Agriculture, Antibiotics, Antimicrobials, Chromatography, Diseases, High performance liquid chromatography, Indicators, Mass spectrometry, Separation, Size-exclusion chromatography, Solid phase extractions, Solvents

Aminoglycoside Analysis in Pork Muscle using Molecularly Imprinted Polymer Cleanup and LC/MS/MS Detection

Introduction Aminoglycosides are a well-known class of antibiotics that are routinely monitored in animal-derived foods. Many countries have instituted regulatory limits for aminoglycosides. There is...
Emily Barrey, Olga Shimelis, K. G. Espenschied
Reporter US, Volume 32.2
Keywords: Agriculture, Antibiotics, Food Safety, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Separation, Size-exclusion chromatography, Solid phase extractions

Peer-Reviewed Papers

References

Set your institution to view full text papers.

1. High frequency transfer and horizontal spread of apramycin resistance in calf faecal Escherichia coli. C. M. Yates, M. C. Pearce, et. Al J. Antimicrob. Chemother. 54, 534-537, (2004)

Loading...


2. In Vitro Model of Colonization Resistance by the Enteric Microbiota: Effects of Antimicrobial Agents Used in Food-Producing Animals. R. Doug Wagner, Shemedia J. Johnson, et al. Antimicrob. Agents Chemother. 52, 1230-1237, (2008)

Loading...


3. Apramycin recognition by the human ribosomal decoding site. 1.Hermann T, Tereshko V, et al. Blood Cells Mol. Dis. 38, 193-198, (2007)

Loading...


4. Effects of apramycin, a novel aminoglycoside antibiotic on bacterial protein synthesis. Perzynski, S., et al. Eur. J. Biochem. 99, 623, (1979)

Loading...


Dissociation of antibacterial activity and aminoglycoside ototoxicity in the 4-monosubstituted 2-deoxystreptamine apramycin. Matt T, Ng CL, Lang K, et al. Proc. Natl. Acad. Sci. U. S. A. 109(27), 10984-9, (2012)

Loading...


Correlation between apramycin and gentamicin use in pigs and an increasing reservoir of gentamicin-resistant Escherichia coli. Jensen VF, Jakobsen L, Emborg HD, et al. J. Antimicrob. Chemother. 58(1), 101-7, (2006)

Loading...


The impact of aminoglycosides on the dynamics of translation elongation. Tsai A, Uemura S, Johansson M, et al. Cell Rep. 3(2), 497-508, (2013)

Loading...


Crystallographic studies of Homo sapiens ribosomal decoding A site complexed with aminoglycosides. Kondo J, François B, Urzhumtsev A, et al. Nucleic Acids Symp. Ser. (49), 253-4, (2005)

Loading...


Aminoglycoside binding to the HIV-1 RNA dimerization initiation site: thermodynamics and effect on the kissing-loop to duplex conversion. Bernacchi S, Freisz S, Maechling C, et al. Nucleic Acids Res. 35(21), 7128-39, (2007)

Loading...


Differential selectivity of natural and synthetic aminoglycosides towards the eukaryotic and prokaryotic decoding A sites. Kondo J, Hainrichson M, Nudelman I, et al. ChemBioChem. 8(14), 1700-9, (2007)

Loading...


Effects of supplementation of beta-glucans on growth performance, nutrient digestibility, and immunity in weanling pigs. Hahn TW, Lohakare JD, Lee SL, et al. J. Anim. Sci. 84(6), 1422-8, (2006)

Loading...


Novel apramycin resistance gene apmA in bovine and porcine methicillin-resistant Staphylococcus aureus ST398 isolates. Andrea T Fessler et al Antimicrob. Agents Chemother. 55, 373-5, (2011)

Loading...


Beta-glucuronidase as a sensitive and versatile reporter in actinomycetes. Myronovskyi M, Welle E, Fedorenko V, et al. Appl. Environ. Microbiol. 77(15), 5370-83, (2011)

Loading...


Unusual small plasmids carrying the novel resistance genes dfrK or apmA isolated from methicillin-resistant or -susceptible staphylococci. Kadlec K, Feßler AT, Couto N, et al. J. Antimicrob. Chemother. 67(10), 2342-5, (2012)

Loading...


Field trial evaluating changes in prevalence and patterns of antimicrobial resistance among Escherichia coli and Enterococcus spp. isolated from growing broilers medicated with enrofloxacin, apramycin and amoxicillin. da Costa PM, Belo A, Gonçalves J, et al. Vet. Microbiol. 139(3-4), 284-92, (2009)

Loading...


Enhancement of bacterial competitive fitness by apramycin resistance plasmids from non-pathogenic Escherichia coli. Yates CM, Shaw DJ, Roe AJ, et al. Biol. Lett. 2(3), 463-5, (2006)

Loading...


Apramycin and gentamicin resistances in indicator and clinical Escherichia coli isolates from farm animals in Korea. Choi MJ, Lim SK, Nam HM, et al. Foodborne Pathog. Dis. 8(1), 119-23, (2011)

Loading...


Resistance patterns and detection of aac(3)-IV gene in apramycin-resistant Escherichia coli isolated from farm animals and farm workers in northeastern of China. Zhang XY, Ding LJ, and Fan MZ Res. Vet. Sci. 87(3), 449-54, (2009)

Loading...


Glycine origin of the methyl substituent on C7'-N of octodiose for the biosynthesis of apramycin. Xu M, Zhu Y, Jin Z, et al. Sci. China,. C, Life Sci. 49(4), 362-9, (2006)

Loading...


Structural features and oxidative stress towards plasmid DNA of apramycin copper complex. Balenci D, Bonechi G, D'Amelio N, et al. Dalton Trans. (7), 1123-30, (2009)

Loading...


Evaluation of a survey approach to estimating the prevalence of cattle carrying antimicrobial-resistant Escherichia coli. Gunn GJ, Hall M, Hunter EA, et al. Vet. J. 175(3), 416-8, (2008)

Loading...


Marker removal from actinomycetes genome using Flp recombinase. Fedoryshyn M, Petzke L, Welle E, et al. Gene 419(1-2), 43-7, (2008)

Loading...


Synthesis, separation, and characterization of amphiphilic sulfated oligosaccharides enabled by reversed-phase ion pairing LC and LC-MS methods. Fenner AM and Kerns RJ Carbohydr. Res. 346(17), 2792-800, (2011)

Loading...


Determination of apramycin in animal feeds by solid-phase extraction and liquid chromatography with precolumn derivatization and fluorescence detection. Zhang L, Wang Z, Chang B, et al. J. AOAC Int. 90(4), 885-91, (2007)

Loading...


A new post-PKS modification process in the carbamoyltransferase gene inactivation strain of Streptomyces hygroscopicus 17997. Li Y, He W, Wang Y, et al. J. Antibiot. 61(6), 347-55, (2008)

Loading...


Structural features of apramycin bound at the bacterial ribosome a site as detected by NMR and CD spectroscopy. Balenci D, D'Amelio N, Gaggelli E, et al. ChemBioChem. 11(2), 166-9, (2010)

Loading...


Detection and characterization of aparmycin-resistant Escherichia coli from humans in Korea. Lim SK, Nam HM, Kim A, et al. Microb. Drug Resist. 17(4), 563-6, (2011)

Loading...


Engineering Streptomyces tenebrarius to synthesize single component of carbamoyl tobramycin. Hong W and Yan S Lett. Appl. Microbiol. 55(1), 33-9, (2012)

Loading...


Related Products

related product

Product #

Description

Add to Cart

08168 Timestrip Plus 8 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?