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A204 Sigma

Anti-Epidermal Growth Factor Receptor antibody produced in sheep

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-EGFR

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Cytokines and Growth Factors,
species reactivity   rat, dog, human, chicken
application(s)   immunocytochemistry: 1:50-1:200 using formalin-fixed cells
  immunofluorescence: suitable
  immunohistochemistry: suitable
  immunoprecipitation: 10-12 μg/mL using A-431 epidermoid carcinoma cell extracts.
  western blot: 1:50-1:200
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... EGFR(1956)
rat ... Egfr(24329)
biological source   sheep
conjugate   unconjugated

Description

Immunogen

fusion protein (20 amino acid residues) of the human EGF receptor. The sequence is proximal to the phosphorylation region (near the N-terminal sequence).

General description

EGFR is a glycoprotein receptor that consists of 3 domains: an extracellular domain which modulates ligand binding and receptor dimerization, a single transmembrane domain, and a cytoplasmic domain. EGFR/ErbB1 binds to EGF ligands and is known to form homodimers or heterodimers. Furthermore, EGFR regulates differentiation, growth, movement and apoptosis in a wide range of cells.
Sheep anti-EGFR recognizes rat, mouse, dog, chicken and human EGF receptors. Furthermore, the antibody recognizes the internal domain of the receptor molecule and will block the phosphorylation but will not block EGF binding. The antibody can be used with formalin-fixed, paraffin-embedded tissue sections.

Physical form

0.2 μm filtered solution in 0.15 M phosphate buffered saline, pH 7.5.

Preparation Note

Purified by protein G affinity chromatography.

Application

Sheep anti-EGFR antibody was used to detect EGFR by immunofluorescence in mouse oocyte cumulus cells that were previously fixed in 4% paraformaldehyde

Sheep anti-epidermal growth factor receptor (EGFR) can be used for immunohistochemistry, immunoprecipitation, western blot and immunocytochemistry applications.

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