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A2050 Sigma

Avidin–FITC from egg white

buffered aqueous solution

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Properties

Related Categories Antibodies, Avidin Reagents, Biotin/Avidin, Supplementary Products
form   buffered aqueous solution
extent of labeling   3-5.5 mol fluorochrome per mol protein
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20-40 μg/mL
  indirect immunofluorescence: 1:200
conjugate   FITC conjugate
shipped in   dry ice
storage temp.   −20°C
Gene Information   chicken ... AVD(396260)

Description

Preparation Note

Affinity purified protein.

Physical form

Solution in 0.01 M phosphate buffered saline containing 10% (v/v) 0.5 M carbonate buffer, pH 9.5, and 15 mM sodium azide.

Application

Avidin-FITC from egg white can be used for immunohistochemistry in formalin-fixed, paraffin-embedded sections at a concentration of 20-40 μg/mL. The product can also be used for indirect immunofluorescence (1:200).

To determine the amount of labeled polyclonal IgG present in spores in an IgG prep. A known concentration of FITC avidin from egg white and biotin-IgG avidin was used in excess at 300nm.

General description

Avidin is homotetrameric protein (68 kDa) that is obtained from egg whites. This egg protein binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.

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Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg
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