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A2052 Sigma

Anti-GABA antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-γ-Aminobutyric acid

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Neural Stem Cells, Antibodies for Neurotransmission,
biological source   rabbit
antibody form   affinity isolated antibody
clone   polyclonal
form   buffered aqueous solution
species reactivity   wide range, rat, Drosophila
application(s)   dot blot: 1:10,000
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2.5 μg/mL using rat cerebellum
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

γ-aminobutyric acid (GABA) conjugated to BSA.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Application

Expression of GABA in neucortical cells harvested from the brains of E19 day old rat embyros was detected by immunofluorescence using rabbit anti-GABA antibody. Triple IF staining was performed with the anti-GABA antibody and two anti-GAD antibodies. Expression of GABA was analyzed in cells isolated from the pallium of various animals including rats, mice, rabbits, guinea pigs, and lizards by immunohistochemistry. IHC was performed using rabbit anti-GABA antibody at 1:1000 diluted in a solution of 0.01M PBS ph 7.4 + 0.5% triton-x100.

Rabbit anti-GABA antibody has been used for electron microscopy analysis in rat hippocampal tissues at a dilution of 1:4000. The antibody has also been used for immunocytochemistry applications at dilutions ranging from 1:100-1:750 in Drosophila and Periplaneta americana brain cells.

General description

GABA (γ-Aminobutyric acid) is the major inhibitory neurotransmitter in the central nervous system and also functions as a neuromodulator in some peripheral tissues. GABA is present in non-nervous structures, and plays an essential role in the modulation of heart rate decreases, blood pressure, and respiratory depression. GABA facilitates neuronal inhibition by interacting with GABA/benzodiazepine receptor and opening an integral chloride channel. Rabbit anti-GABA antibody shows positive binding with GABA and GABA-KLH in a dot blot assay, and exhibits negative binding with BSA.

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Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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