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A2066 Sigma

Anti-Actin antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Properties

Related Categories AC-AC, Actin Antibodies, Alphabetical Index, Antibodies, Antibodies for Cell Biology,
species reactivity   amoeba, wide range, chicken, slime mold, vertebrates, human
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25 using human or animal tissue sections
  indirect immunofluorescence: 1:40 using chicken fibroblasts
  western blot: 1:100 using chicken gizzard extract
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt 42 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... ACTA1(58)
mouse ... Acta1(11459)
rat ... Acta1(29437)
conjugate   unconjugated

Description

Immunogen

The C-terminal actin fragment (C11 peptide, Ser-Gly-Pro-Ser-Ile-Val-His-Arg-Lys-Cys-Phe) attached to Multiple Antigen Peptide (MAP) backbone).

General description

Actin is a cytoskeletal protein that regulates cell motility, secretion, phagocytosis and cytokinesis. The NH2-terminal of actin may function as an antigen1,2. This terminal may also modulate actin interactions and may associate with proteins such as myosin.
Anti-Actin specifically stains stress fibers in cultured chicken fibroblasts using indirect immunofluorescence and specifically localizes actin by immunoperoxidase labeling of formalin-fixed, paraffin embedded human or animal tissues. In immunoblotting, the product localizes actin in many species ranging from human skeletal muscle to amoeba. The product recognizes the 42 kDa actin band using immunoblotting techniques in human or animal tissue extracts.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as preservative.

Application

May be used to study actin structure and function and to probe binding sites of actin-binding proteins.

Rabbit anti-actin antibody can be used for western blot assays. The antibody can also be used for immunohistochemistry (1:25, using human or animal tissue sections) and indirect immunofluorescence (1:40, using chicken fibroblasts) applications.

Used to study actin structure and function and to probe binding sites of actin-binding proteins. Protein lysates generated from embryonic mouse limb tissue was analyzed by western blot using actin as the loading control. Actin was detected using rabbit anti-actin at 1:8000. Actin detected by a rabbit anti-actin antibody was used as a loading control for MCF7 cell lysates . Actin was detected at 42 kDa.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

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