A2066 Sigma

Anti-Actin antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories AC-AC, Actin Antibodies, Alphabetical Index, Antibodies, Antibodies for Cell Biology,
species reactivity   amoeba, wide range, slime mold, vertebrates
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25 using human or animal tissue sections
  indirect immunofluorescence: 1:40 using chicken fibroblasts
  western blot: 1:100 using chicken gizzard extract
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt 42 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... ACTA1(58)
mouse ... Acta1(11459)
rat ... Acta1(29437)

Description

Immunogen

The C-terminal actin fragment (C11 peptide, Ser-Gly-Pro-Ser-Ile-Val-His-Arg-Lys-Cys-Phe) attached to Multiple Antigen Peptide (MAP) backbone).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as preservative.

Application

May be used to study actin structure and function and to probe binding sites of actin-binding proteins.

Used to study actin structure and function and to probe binding sites of actin-binding proteins. Protein lysates generated from embryonic mouse limb tissue was analyzed by western blot using actin as the loading control. Actin was detected using rabbit anti-actin at 1:8000. Actin detected by a rabbit anti-actin antibody was used as a loading control for MCF7 cell lysates . Actin was detected at 42 kDa.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

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Price and Availability

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