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A2074 Sigma

Monoclonal Anti-Rabbit Immunoglobulins–Peroxidase antibody produced in mouse

clone RG-16, purified immunoglobulin, lyophilized powder

Synonym: Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM)

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Properties

Related Categories Alphabetical Index, Antibodies, Antibody Conjugates, Molecular Biology, Peroxidase Conjugates,
biological source   mouse
antibody form   purified immunoglobulin
clone   RG-16, monoclonal
form   lyophilized powder
species reactivity   rabbit
does not react with   guinea pig, turkey, rat, goat, canine, feline, bovine, sheep, chicken, horse, human, pig
packaging   vial of 0.5 mL
application(s)   direct ELISA: 1:30,000
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tissue
  western blot (chemiluminescent): 1:160,000
isotype   IgG1
conjugate   peroxidase conjugate
storage temp.   2-8°C

Description

General description

Reacts with an epitope on the heavy chain of rabbit IgG, IgA and IgM, which is sensitive to reduction. No cross-reactivity with human IgG, IgA and IgM.

IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins.
Mouse monoclonal anti-rabbit immunoglobulins binds to an epitope on the heavy chain of rabbit IgG, IgM, and IgA. Reduction of rabbit immunoglobulins appears to destroy the epitope. The antibody does not cross-react with human serum or tissue components. Furthermore, the antibody does not bind to IgGs from human, guinea pig, rat, bovine, turkey, goat, sheep, horse, dog, chicken, pig, and cat.

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Specificity

Binds only rabbit Igs. Does not react with reduced rabbit Igs.

Physical form

Lyophilized from 0.01 M sodium phosphate buffer, pH 7.4, containing 1% bovine serum albumin and 0.01% thimerosal

Application

ELISA assays on rabbit serum were performed using anti-rabbit IgG-horseradish peroxidase-mouse monoclonal antibody diluted 1:10,000 in blocking buffer and added at 100 μl/well.

Mouse monoclonal anti-rabbit immunoglobulins have been used for western blot assays at a dilution of 1:1000 using bovine trabecular meshwork cell lysates. The antibody can also be used for immunohistochemistry assays (1:100, using human tissue).

The mouse monoclonal anti-Rabbit IgG (heavy chain-specific) peroxidase conjugated antibody can be used as a secondary antibody for western blot analysis of protein preps from E. coli cultures at a concentration of 1:10000 diluted in PBSt for 1 hour at room temperature.

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Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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Antibody Explorer - Over 80,000 Products

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

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