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A2080 Sigma

Monoclonal Anti-Alkaline Phosphatase, Human Placental−Agarose antibody produced in mouse

clone 8B6, purified immunoglobulin, PBS suspension

Synonym: Anti-Phosphatase, Alkaline, Human Placental, Monoclonal Anti-Alkaline Phosphatase, Human Placental, PLAP

Properties

Related Categories AL-AL, Alkaline Phosphatase Detection, Alphabetical Index, Antibodies, Antibodies to Peptide and Protein Fusion Tags,
species reactivity   human
application(s)   immunoprecipitation: suitable
clone   8B6, monoclonal
antibody form   purified immunoglobulin
form   PBS suspension
isotype   IgG2a
shipped in   wet ice
storage temp.   2-8°C
Gene Information   human ... ALPP(250)
biological source   mouse
extent of labeling   2 mg antibody per mL bed volume
conjugate   agarose conjugate

Description

Immunogen

Human epidermoid carcinoma cell line expressing placental alkaline phosphatase.

General description

Alkaline phosphatases are non-specific phosphomonoesterases that function optimally at alkaline pH. Placental alkaline phosphatase differs from the alkaline phosphatases of bone, liver and kidney, in the hydrolysis rate of various phosphate-based substrates, and also in its degree of inhibition by L-phenylalanine and L-homoarginine or levamisole. Human Placental Alkaline Phosphatase (hPLAP) has been studied as a potential tumor marker.

In SDS gels, the product reacts with both Regan and Nagao isozymes of human placental alkaline phosphatase (hPLAP, 130 kDa, 67/130 kDa). By RIA, the antibody binds to hPLAP with an affinity constant of 5 × 109 LM-1. It does not react with PLAP-like enzymes.

Physical form

Suspension in 0.1 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Prepared using purified antibody coupled to cyanogen bromide-activated agarose.

Application

Anti-Alkaline phosphatase human placental antibody coupled to agarose was packed in a column and used to purify alkaline-phosphatase conjugated FGF receptor out of conditioned media from COS-7 cells. Column was washed with 1M NaCl.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Cultured pancreatic islet cells were fixed with cold 4% paraformaldhyde at room temperature for 10 minutes and incubated with mouse monoclonal anti-Alkaline Phosphatase, Human Placental antibody at 1:500. Mouse monoclonal anti-Alkaline Phosphatase, Human Placental antibody was used to detect a unique molecular probe that consisted of the FGFR extracellular domain expressed as a chimera with human PLAP.

This immunoadsorbant may be used to purify fusion proteins produced using vectors expressing the hPLAP gene. Suitable for affinity purification and immunoprecipitation procedures of human placental alkaline phosphatase either natural or recombinant.

Packaging

1 mL in glass bottle

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