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A2095 Sigma

Monoclonal Anti-HA−Agarose antibody produced in mouse

clone HA-7, purified immunoglobulin, PBS suspension

Synonym: Anti-HA, Anti-Influenza Hemagglutinin, Monoclonal Anti-HA

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Properties

Related Categories Additional Affinity Purification, Alphabetical Index, Antibodies, Antibodies to Peptide and Protein Fusion Tags, H1-HE,
biological source   mouse
antibody form   purified immunoglobulin
clone   HA-7, monoclonal
form   PBS suspension
isotype   IgG1
capacity   20-50 nmol/mL elution capacity (HA-tagged fusion protein)
  30-50 nmol/mL binding capacity (HA-tagged fusion protein)
conjugate   agarose conjugate
shipped in   wet ice
storage temp.   2-8°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Suitable for use in immunoprecipitation assays and for immunoaffinity purification of HA-tagged fusion proteins.

Features and Benefits

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Immunogen

synthetic peptide corresponding to amino acid residues 98-106 (YPYDVPDYA) of human Influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH.

Preparation Note

Prepared using cyanogen bromide-activated agarose. Specific antibody concentration is 2.0-2.4 mg/ml settled resin.

Specificity

The antibody recognizes native as well as denatured-reduced forms of HA-tagged proteins and is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells.

Physical form

Suspension in 0.01 M phosphate buffered saline, containing 15 mM sodium azide

General description

Monoclonal Anti-HA Agarose Conjugate recognizes native as well as denatured-reduced forms of the HA (hemagglutinin)-tagged proteins and is reactive with N-or C-terminal HA-tagged fusion proteins expressed in E.coli or in mammalian cells.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
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