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A2153 Sigma

Bovine Serum Albumin

lyophilized powder, ≥96% (agarose gel electrophoresis)

Synonym: Albumin bovine serum, BSA, Bovine albumin

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Properties

Related Categories Albumin, Albumins, Biochemicals and Reagents, Bovine Serum Albumins, Core Bioreagents,
assay   ≥96% (agarose gel electrophoresis)
form   lyophilized powder
mol wt   mol wt ~66 kDa
purified by   cold ethanol fractionation
packaging   poly bottle of
origin   USA origin
loss   ≤6%
pH   6.5-7.5 (1% in 0.15 M sodium chloride)
  7
solubility   water: soluble (40 mg/ml)
storage temp.   2-8°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Packaging

1, 5 kg in poly drum

10, 50, 100, 500 g in poly bottle

Preparation Note

Often referred to as Cohn fraction V; this product is prepared by a modified method of the Cohn cold ethanol fractionation method.

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Application

Bovine serum albumin has been used-
• along with BTS (Beltsville thawing solution), for the extension of sperms during sperm motion analysis
• as a component of modified Krebs-Ringer buffer (dmKRBT)
• in gel shift analysis

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

Is Product A2153, Albumin from bovine serum (BSA), suitable for use in ELISA?
We have not tested A2153 in quality control for use in ELISA applications. Because it contains globulins, we would generally not recommend A2153 for use in ELISA.
Is Product A2153, Albumin from bovine serum (BSA), suitable for use in immunoblotting experiments as a blocking agent?
We have not tested A2153 in quality control for use in immunoblotting applications as a blocking agent. Because it contains globulins, we would generally not recommend A2153 for use as a blocking agent.
Product A2153, Albumin from bovine serum (BSA), powder looks tan in color.  Is this OK?
The tan color is within specifications. The color can vary because of factors such as concentration of a solution at the time of lyophilization.
Is Product A2153, Albumin from bovine serum (BSA), suitable for cell culture?
We do not test A2153 for cell culture use, or for any specifications such as endotoxin content. Customers will have to exercise their own judgment as to whether they wish to use A2153 in cell culture, or can search the literature for publications that cite this product number.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
I need help selecting an Albumin from bovine serum (BSA) product, is there a guide to help me choose the best product?
Yes,  a selection guide to our BSA products can be found at the link below: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/General_Information/albumin_application_selection_guide1.Par.0001.File.tmp/albumin_application_selection_guide1.pdf
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Protocols & Articles

Articles

HPLC Analysis of Proteins on SRT® SEC-150, 30 cm x 7.8 mm I.D., 5 μm particles

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on SRT® SEC-150, 30 cm x 7.8 mm I.D., 5 μm particles
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Proteins on SRT® SEC-300, 30 cm x 7.8 mm I.D., 5 μm

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on SRT® SEC-300, 30 cm x 7.8 mm I.D., 5 μm
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Proteins on SRT® SEC-500, 30 cm x 7.8 mm I.D.

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on SRT® SEC-500, 30 cm x 7.8 mm I.D.
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

Peer-Reviewed Papers
15

References

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