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A2189 Sigma

Monoclonal Anti-Human IgM (μ-chain specific)−Alkaline Phosphatase antibody produced in mouse

clone MB-11, purified immunoglobulin, buffered aqueous glycerol solution

Synonym: Monoclonal Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase

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Properties

Related Categories Alkaline Phosphatase, Alphabetical Index, Antibodies, HU-HZ, Human IgM Secondary Antibodies and Conjugates,
biological source   mouse
antibody form   purified immunoglobulin
clone   MB-11, monoclonal
form   buffered aqueous glycerol solution
species reactivity   human
application(s)   direct ELISA: 1:35,000
  dot blot: 1:20,000 using 20 ng of human IgM/dot
  dot blot: 1:30,000 using 20 ng of human IgM/dot
isotype   IgG2b
conjugate   alkaline phosphatase conjugate
shipped in   wet ice
storage temp.   2-8°C

Description

Immunogen

Human IgM isolated from pooled normal human serum

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide

Application

Monoclonal Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase antibody is suitable for use in ELISA (1:1000). This product may also be used for dot blot (1:20,000) and chemiluminescent western blot (1:30,000).

General description

Human IgMs are the initial immunoglobulin isotypes that appear in blood in response to the first exposure to external antigens. IgMs have been implicated in CNS myelin repair and increased secretory IgM levels have been linked to rhino-conjunctivitis . Monoclonal Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase antibody is specific for human IgM. The product does not react with human IgG, IgA or light chains.

Price and Availability


Available in ELITE Grade
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

Related Content

Antibody Explorer - Over 80,000 Products

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

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