A2230 Sigma

Apyrase from potatoes

High Activity, ATPase ≥600 units/mg protein, lyophilized powder

Synonym: Adenosine 5′-diphosphatase, Adenosine 5′-triphosphatase

Properties

Related Categories 3.6.x.x Acting on acid anhydrides, 3.x.x.x Hydrolases, Biochemicals and Reagents, Enzyme Class Index, Enzymes, Inhibitors, and Substrates More...
Quality Level   PREMIUM
form   lyophilized powder
quality   High Activity
secondary activity   ≥50 % of base activity ADPase
composition   protein, ≥30%
foreign activity   Acid Phosphatase ≤2% of base activity
shipped in   wet ice
storage temp.   −20°C

Description

Application

At least two isoenzymes are found in different varieties of S. tuberosum:3,4 one with a high ATPase/ADPase ratio (∼10) and another with a low ratio (∼1).
Reaction: ATP → ADP+Pi → AMP+2Pi.

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation 1. Product A2230 is a high activity apyrase.

Packaging

Sold on the basis of ATPase units.

Preparation Note

Derived from red potato

Unit Definition

One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.

Physical form

Lyophilized powder containing potassium succinate buffer salts.

Biochem/physiol Actions

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis 2. Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.

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