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A2306 Sigma

Monoclonal Anti-Rabbit Immunoglobulins−Alkaline Phosphatase antibody produced in mouse

clone RG-16, purified immunoglobulin, buffered aqueous glycerol solution

Synonym: Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM)



Related Categories Alkaline Phosphatase, Alkaline Phosphatase Conjugates, Alphabetical Index, Antibodies, Antibody Conjugates,
biological source   mouse
antibody form   purified immunoglobulin
clone   RG-16, monoclonal
form   buffered aqueous glycerol solution
species reactivity   rabbit
does not react with   guinea pig, turkey, rat, goat, canine, feline, bovine, sheep, chicken, horse, human, pig
application(s)   direct ELISA: 1:40,000
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160 using human tonsil and Cat. No. I8635 as the primary antibody
  western blot: 1:160,000-1:320,000 using detecting β-actin in total cell extract of HeLa cells (5-10 μg per well)
isotype   IgG1
conjugate   alkaline phosphatase conjugate
shipped in   wet ice
storage temp.   2-8°C


General description

Reacts with an epitope on the heavy chain of rabbit IgG, IgA and IgM, which is sensitive to reduction. No cross-reactivity with human IgG, IgA and IgM.

IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins.
Mouse monoclonal anti-rabbit immunoglobulins-alkaline phosphatase antibody binds to an epitope on the heavy chain of rabbit IgG, IgA, and IgM. The product stains bands at the intact whole molecule and at the heavy chains in an immunoblot of denatured, non-reduced rabbit immunoglobulins. Reduction of rabbit immunoglobulins destroys the epitope. In ELISA, the product shows no cross reaction with human serum or tissue preparations, nor with human IgA, IgG, or IgM. No cross reaction is observed with IgG from the following species: bovine, cat, chicken, dog, goat, guinea pig, horse, pig, rat, sheep or turkey.


Binds only rabbit Igs. Does not react with reduced rabbit Igs.

Physical form

Solution in 0.05 M Tris buffer pH 8.0, containing 1 mM MgCl2, 0.1% bovine serum albumin, 50% glycerol and 15 mM sodium azide


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Mouse monoclonal anti-rabbit immunoglobulins-alkaline phosphatase antibody can be used for direct ELISA (1:40,000) and immunohistochemistry (using formalin-fixed, paraffin-embedded sections at 1:160 dilution).

The mouse monoclonal anti-Rabbit IgG (heavy chain-specific) peroxidase conjugated antibody can be used as a secondary antibody for western blot analysis of protein preps from E. coli cultures at a concentration of 1:10000 diluted in PBSt for 1 hour at room temperature.

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NONH for all modes of transport
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