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A2411 Sigma

Amphotericin B from Streptomyces sp.

BioReagent, suitable for cell culture, ~80% (HPLC)

Synonym: Fungizone

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Properties

Related Categories Antibiotic powders, Antibiotics, Antibiotics A to Z, Antibiotics A-F, Antifungal,
Quality Level   PREMIUM
biological source   from Streptomyces sp.
material   yellow to yellow orange
InChI Key   APKFDSVGJQXUKY-INPOYWNPSA-N
product line   BioReagent
assay   ~80% (HPLC)
form   solid
potency   >750.00 μg per mg (Dry basis)
composition   (%C anhydrous)
, 58.20-65.00%
  (%N anhydrous)
, 1.20-1.80%
solubility   H2O: slightly soluble
suitability   suitable for cell culture
Mode of action   cell membrane | interferes
antibiotic activity spectrum   fungi
  yeast
storage temp.   2-8°C

Description

Application

Amphotericin B is a polyene antifungal antibiotic from Streptomyce sp. It has a high affinity for sterols, especially ergosterols, of both fungal and bacterial cell membranes. Normal usage in maintenance of antifungal cell cultures is 2.5 mg/L with penicillin and streptomycin used in the medium.

Biochem/physiol Actions

Amphotericin B binds to sterols, forming pores in the membrane, and causing small molecules to leak out. The product is most effective against fungi and yeast.

Caution

Amphotericin B remains active for 3 days in culture at 37°C. At -20°C, and with protection from air and light, the product remains active for 5 years.

Packaging

1, 5 g in glass bottle

250 mg in glass bottle

Preparation Note

Amphotericin B is insoluble in water at pH 6 to 7, but soluble in water at pH 2 or 11. It is soluble in DMSO (30-40 mg/mL) and in dimethylformamide (2-4 mg/mL). Aqueous solutions cannot be sterile filtered due to poor solubility.

Protocols & Applications

Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.



Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
BU2625000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Related Content

Antibiotic Selection Guide

1Application Key: CC = Callus Culture; PR = plant regeneration; EC = cotyledon, hypocotyl, or leaf disc culture; TS = transformant selection; SE = somatic embryogenesis; SSC = stem selection culture;...
Keywords: Antibiotics, Cell culture

Peer-Reviewed Papers
15

References

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