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A2411 Sigma

Amphotericin B from Streptomyces sp.

BioReagent, suitable for cell culture, ~80% (HPLC)

Synonym: Fungizone

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Properties

Related Categories Antibiotic powders, Antibiotics, Antibiotics A to Z, Antibiotics A-F, Antifungal,
Quality Level   PREMIUM
biological source   from Streptomyces sp.
InChI Key   APKFDSVGJQXUKY-INPOYWNPSA-N
product line   BioReagent
assay   ~80% (HPLC)
form   solid
potency   >750.00 μg per mg (Dry basis)
color   yellow to yellow-orange
solubility   H2O: slightly soluble
suitability   suitable for cell culture
Mode of action   cell membrane | interferes
antibiotic activity spectrum   fungi
  yeast
storage temp.   2-8°C

Description

Application

Amphotericin B is a polyene antifungal antibiotic from Streptomyce sp. It has a high affinity for sterols, especially ergosterols, of both fungal and bacterial cell membranes. Normal usage in maintenance of antifungal cell cultures is 2.5 mg/L with penicillin and streptomycin used in the medium.

Biochem/physiol Actions

Amphotericin B binds to sterols, forming pores in the membrane, and causing small molecules to leak out. The product is most effective against fungi and yeast.

Caution

Amphotericin B remains active for 3 days in culture at 37°C. At -20°C, and with protection from air and light, the product remains active for 5 years.

Packaging

1, 5 g in glass bottle

250 mg in glass bottle

Preparation Note

Amphotericin B is insoluble in water at pH 6 to 7, but soluble in water at pH 2 or 11. It is soluble in DMSO (30-40 mg/mL) and in dimethylformamide (2-4 mg/mL). Aqueous solutions cannot be sterile filtered due to poor solubility.

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Product is sensitive to light and moisture.

Protocols & Applications

Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

Price and Availability


Laboratory Gloves
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
BU2625000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Related Content

Antibiotic Selection Guide

1Application Key: CC = Callus Culture; PR = plant regeneration; EC = cotyledon, hypocotyl, or leaf disc culture; TS = transformant selection; SE = somatic embryogenesis; SSC = stem selection culture;...
Keywords: Antibiotics, Cell culture

Peer-Reviewed Papers
15

References

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