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A25120 Sigma

DEAE–Sephadex® A-25 chloride form

Synonym: DEAE Sephadex®, Diethylaminoethyl Sephadex®



Related Categories Analytical/Chromatography, Anion Exchange Media, Ion Exchange Chromatography, Molecular Biology, Protein Chromatography,
bead size   40-125 μm (dry)
pore size   ~30,000 Da exclusion limit
operating pH   2 - 9
capacity   3-4 meq/g ion exchange capacity
compatibility   mode of use mixed weak and strong anion exchange chromatography


Frequently Asked Questions

Frequently Asked Questions are available for this Product.


10, 50, 100, 500 g in poly bottle


DEAE Sephadex can be used in ion exchange chromatography for purifying and isolating proteins. It has been used to identify and label an insulin activated nitric oxide synthase inhibitor (protein) in acute myocardial infarction (AMI) patients.

Legal Information

DEAE Sephadex is a registered trademark of GE Healthcare

Sephadex is a registered trademark of GE Healthcare

Price and Availability

Amplified Detection
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How should I store Product A25120, DEAE–Sephadex® A-25 chloride?
Unused media can be stored in the container at +4 to +30 °C.  Ensure that the screw-top is fully tightened.  Packed columns should be equilibrated in working buffer containing 20% ethanol to prevent microbial growth.
What is the chemical stability of Product A25120, DEAE–Sephadex® A-25 chloride form?  
According to the manufacturer, this is compatible with all commonly used aqueous buffers, 1 M NaOH, 8 M urea, 6 M guanidine hydrochloride and 70% ethanol.
How do I equilibrate Product A25120, DEAE–Sephadex® A-25 chloride form?
After packing, and before a chromatographic run, equilibrate with working buffer by washing with at least 5 bed volumes.
How should I regenerate Product A25120, DEAE–Sephadex® A-25 chloride form?
After each separation, elute any reversibly bound material either with a high ionic strength solution (e.g. 1 M NaCl in buffer) or by increasing pH. Regenerate the media by washing with at least 5 bed volumes of buffer, or until the column effluent shows stable conductivity and pH values.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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