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A2529 Sigma

Alcohol dehydrogenase–Agarose from baker's yeast (S. cerevisiae)

lyophilized powder

Synonym: ADH, Alcohol:NAD+ oxidoreductase

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Description

Packaging

25 units in poly bottle

Unit Definition

One unit will convert 1.0 μmole of ethanol to acetaldehyde per min at pH 8.8 at 25 °C.

Application

Alcohol dehydrogenase may be used to synthesize enantiomerically pure stereoisomers of chiral alcohols. It may be used to study ethanol fuel cells, alcoholism and drug dependence . Product A2529 is from S. Cerevisiae and is an insoluble enzyme with endless applications.

Biochem/physiol Actions

Sigma insoluble enzymes are produced by reacting a soluble enzyme with an inert base. This produces an insoluble compound with the activity of the original enzyme. Alcohol dehydrogenase has broad substrate specificity for alcohols, ketones and acetaldehyde.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Protocols

Enzymatic Assay Of Alcohol Dehydrogenase Attached To Agarose (A2529)

The objective of this procedure is to standardize the enzymatic assay of Alcohol Dehydrogenase attached to Agarose, Sigma Product Number (A2529) , at Sigma-Aldrich St. Louis.
Keywords: Extinction coefficient

Enzymatic Assay of Alcohol Dehydrogenase (EC 1.1.1.1)

This procedure may be used for Alcohol Dehydrogenase products, except for Alcohol Dehydrogenase, Insoluble Enzyme attached to beaded agarose (Catalog No. A2529).
Keywords: Extinction coefficient

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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