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A2543 Sigma

Anti-α-Actinin antibody produced in rabbit

whole antiserum

Properties

Related Categories AC-AC, Alphabetical Index, Antibodies, Antibodies for Cardiac Stem Cells, Antibodies for Cell Biology,
species reactivity   chicken
application(s)   indirect immunofluorescence: 1:500 using cultured chicken fibroblasts
  western blot: suitable using cultured cells and chicken gizzard extract
clone   polyclonal
antibody form   whole antiserum
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   chicken ... ACTN2(396263), ACTN4(396024)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

α-actinin from chicken gizzard

General description

α-Actinin is a 100kDa actin-binding protein that is found in muscle as well as non-muscle cells. In smooth muscles, α-actinin is present in dense bodies and plaques whereas in normal skeletal muscles, it is associated with z-discs that define muscle sarcomeres. Rabbit anti-α-actinin antibody stains α-actinin in cell cultures using indirect immunofluorescence. The antibody reacts best with cultured chicken fibroblasts, however, adequate labeling may also be obtained with cells of other species. In an immunoblot assay, the antiserum specifically stains the α-actinin band.

Application

Anti-Actinin antibody was used to detect actinin in human mesangial cells. Cells were first grown on glass cover slips, fixed in cold methanol and permeabilized with 3.5% paraformaldehyde/0.1% Tween-20 for 5 min at room temperature.

Rabbit anti-α-actinin antibody has been used for co-immunoprecipitation studies. The product has also been used for immunoblotting studies using Arabidopsis tissue extracts.

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