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A2547 Sigma

Monoclonal Anti-Actin, α-Smooth Muscle

clone 1A4, ascites fluid

Synonym: SMA

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Properties

Related Categories AC-AC, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Actin Isoforms,
species reactivity   rabbit, mouse, guinea pig, chicken, goat, sheep, snake, human, frog, rat, canine, bovine
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using smooth muscle cells
  immunohistochemistry (frozen sections): suitable using smooth muscle cells
  indirect immunofluorescence: 1:400 using blood vessels in sections of human appendix
  western blot: suitable using smooth muscle cells
clone   1A4, monoclonal
antibody form   ascites fluid
isotype   IgG2a
mol wt   antigen mol wt ~42 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... ACTA2(59)
mouse ... Acta2(11475)
rat ... Acta2(81633)
biological source   mouse
conjugate   unconjugated
Featured Industry   Research Pathology

Description

Immunogen

N-terminal synthetic decapeptide of α-smooth muscle actin.

General description

Actins are cytoskeletal proteins that regulate cell motility. Several scaffolding, signaling and actin-binding proteins regulate the interconversions between filamentous F-actins and monomeric G-actins. Cellular actins of various species have very similar immunological and physical properties. Hence, the use of monoclonal antibody to actin provides a specific and useful tool for studying the localization, structure and function of actin proteins.
Mouse monoclonal anti-actin, alpha-smooth muscle antibody binds to actin from human, mouse, rat, bovine, chicken, frog, goat, guinea pig, rabbit, dog, sheep, and snake.

The antibody (also known as anti-α-Sm-1) is specific for the single isoform of α-smooth muscle actin. It reacts specifically with α-smooth muscle actin in immunoblotting assays and labels smooth muscle cells in frozen or formalin-fixed, paraffin-embedded tissue sections.

Application

Immunocytochemistry was performed on smooth muscle cells from bovine aortas using the monoclonal anti-ACTA2 antibody. Cells were first grown on glass cover slips and fixed in 50% acetone/EtOH for 10 minutes at 4 degrees.

Mouse monoclonal anti-actin, alpha-smooth muscle antibody has been used for western blot assays using whole kidney homogenates. The product has also been used for immunohistochemical assays using mice tissue sections.

Paraffin embedded sections of rat testis tissue grafts were immunohistochemically stained with mouse monoclonal anti-smooth muscle actin.
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

Articles

Antibodies to Actin Gene Family Isoforms

The Actin Gene Family Actin and myosin are the two major cytoskeletal proteins implicated in cell motility. As constituents of many cell types, actin and myosin are involved in a myriad of cellular p...
Keywords: Western blot

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Peer-Reviewed Papers
15

References

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