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A2554 Sigma

Anti-Mouse IgG (Fc specific)–Peroxidase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibody Conjugates, MO-MR, Molecular Biology,
biological source   goat
antibody form   affinity isolated antibody
clone   polyclonal
form   buffered aqueous solution
species reactivity   mouse
does not react with   human, horse, bovine
application(s)   direct ELISA: 1:70,000
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
  western blot (chemiluminescent): 1:80,000-1:160,000 using total cell extract of Hela cells (5-10 μg/mL)
conjugate   peroxidase conjugate
shipped in   dry ice
storage temp.   −20°C

Description

Other Notes

Antibody adsorbed with bovine, equine and human serum proteins.

Preparation Note

Adsorbed to reduce background staining with bovine, horse, or human samples.

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.01% thimerosal.

Application

Goat anti-mouse IgG (Fc specific)-peroxidase antibody can be used for immunoblotting studies using bovine whole muscle extracts and porcine tissues. Furthermore, the antibody can also be used for western blotting assays using bovine tissue homogenates.

Specificity of a new antibody for PND in blood from immunized mice was tested by Elisa using biotynlated PND and streptavidin coated plates. HRP conjugated goat anti-mouse IgG (Fc specific) was used as secondary.

General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.
Goat anti-mouse IgG(Fc specific)-peroxidase antibody is specific for mouse IgG and mouse IgG, Fc fragment. The conjugate shows no reactivity with mouse Fab fragment, human IgG, IgA, IgM, kappa and lambda light chain, bovine IgG and IgM, or horse IgG.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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