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  • A2582 - Anti-Thioredoxin–Agarose antibody produced in rabbit

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A2582 Sigma

Anti-Thioredoxin–Agarose antibody produced in rabbit

IgG fraction of antiserum, PBS suspension

Synonym: Anti-Thioredoxin

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Properties

Related Categories Additional Affinity Purification, Alphabetical Index, Antibodies, Antibodies to Peptide and Protein Fusion Tags, IP / co-IP,
biological source   rabbit
antibody form   IgG fraction of antiserum
clone   polyclonal
form   PBS suspension
mol wt   antigen mol wt 11.7  kDa
species reactivity   E. coli
conjugate   agarose conjugate
shipped in   wet ice
storage temp.   2-8°C

Description

Immunogen

recombinant E. coli thioredoxin.

Preparation Note

Prepared using antibody coupled to cyanogen bromide activated agarose at 5 mg antibody per mL resin volume.

Specificity

Specific for natural E. coli and recombinant thioredoxin. It may be used to identify and purify the expression of thioredoxin fusion proteins.

Physical form

Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Immunofluorescence was carried out on the cerivcal cancer cell lines SiHa, CaSki, and HeLa using an antibody against the redox proteinThioredoxin.

General description

Thioredoxin is a 11.7kDa electron transport protein that functions as a hydrogen donor during the enzymatic reduction of ribonucleotides to yield deoxyribonucleotides. The thioredoxin system is also involved in the enzymatic reductions of methionine sulfoxide and sulfate. The gene encoding E. coli thioredoxin has been cloned into several plasmids and serves as a fusion partner for the expression of other cloned genes. Hence, the system is useful for the production of soluble fusion proteins in the E. coli cytoplasm. Furthermore, in several instances, these fusion proteins fold correctly and thus display full biological activity.

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