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A2643 Sigma

α-Amylase from porcine pancreas

Type VII-A, DFP treated, ammonium sulfate suspension, ≥500 units/mg protein (E1%/280)

Synonym: β-N-acetylglucosaminidase porcine placenta, PPA, al1,4 glucan-4-glucanohydrolase,, porcine pancreas α-amylase

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Description

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Unit Definition

One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 6.9 at 20 °C.

Physical form

Suspension in 3.2 M (NH4)2SO4, pH 6.1

General description

Molecular mass: 51-54 kDa.
α-Amylase isolated from porcine pancreas is a glycoprotein. It is a single polypeptide chain of ~475 residues containing two SH groups and four disulfide bridges and a tightly bound Ca2+ necessary for stability. Chloride ions are necessary for activity and stability. The pH range for activity is 5.5 to 8.0, with the pH optimum at 7.

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Safety & Documentation
Protocols & Articles

Protocols

Enzymatic Assay of α-AMYLASE (EC 3.2.1.1)

3.2. Unit Definition - One unit will liberate 1.0 mg of maltose from starch in 3 minutes at pH 6.9 at 20°C.
Keywords: Enzyme activity, Enzymology

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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