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A2672 Sigma

Monoclonal Anti-Albumin antibody produced in mouse

clone HSA-9, ascites fluid

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Properties

Related Categories AL-AL, Alphabetical Index, Antibodies, Antibodies to Human Plasma Proteins, Primary Antibodies,
species reactivity   baboon, rhesus monkey, human
does not react with   donkey, guinea pig, rabbit, bovine, goat, pigeon, chicken, dog, marmoset, cat, gibbon, pig, catfish, mouse, sheep, turkey, hamster, horse
application(s)   indirect ELISA: 1:500
clone   HSA-9, monoclonal
antibody form   ascites fluid
isotype   IgG1
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... ALB(213)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

Human platelets

General description

Albumin is a transport protein that binds a broad range of ligands such as fatty acids, bilirubin, hemin, drugs, amino acids and ions . Albumin may also function as a zinc carrier protein and consequently regulate physiological processes . Monoclonal Anti-Albumin antibody is specific for human serum albumin and shows cross reactivity with rhesus monkey and baboon albumins when tested in an immunoblot procedure under non-reducing conditions. The antibody does not react with bovine, cat, catfish, chicken, dog, donkey, gibbon, goat, guinea pig, hamster, horse, marmoset, mouse, pig, pigeon, rabbit, rat, sheep or turkey serum albumin.

Albumin, the major protein produced by liver cells, represents more than half of the total protein content of human serum. Many other body fluids also contain albumin. Three major functions for serum albumin have been proposed: maintenance of osmotic pressure, transportation of a variety of substances and an endogenous source of amino acids. The primary sites of albumin degradation are not known, but the protein can be metabolized by almost every organ in the body. Determination of serum albumin levels is a widely used screening test in clinical medicine. A decrease in serum albumin levels may indicate disease states such as malnutrition, cirrhosis, nephrotic syndrome, diabetes, gastrointestinal and hepatic diseases, thermal burns and pulmonary disease.

Physical form

The product is provided as ascites fluid with 0.1% sodium azide as a preservative.

Application

Monoclonal Anti-Albumin antibody is suitable for use in indirect ELISA (1:500)

Western blot analysis of monkey retinal protein extracts were performed using the mouse monoclonal anti-albumin antibody at 1:20000 and overnight incubation at 4 degrees. Immunohistochemistry was performed on frozen monkey retina sections with the mouse monoclonal anti-albumin antibody at 1:1000. Uptake of albumin by LLC-PK1 cells was monitored by immunofluorescence using the mouse monoclonal anti-albumin antibody. Cells were first fixed in 4% formaldehyde the incubated with the antibody for 1 hour at room temperature.

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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

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Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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