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  • A2691 - Anti-Human IgA (α-chain specific)−Agarose antibody produced in goat

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A2691 Sigma

Anti-Human IgA (α-chain specific)−Agarose antibody produced in goat

affinity isolated antibody, adsorbed with mouse and rat IgG

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Properties

Related Categories Agarose, Alphabetical Index, Antibodies, HU-HZ, Human IgA Secondary Antibodies and Conjugates,
biological source   goat
antibody form   affinity isolated antibody
clone   polyclonal
form   PBS suspension
species reactivity   human
does not react with   rat, mouse
capacity   3-4 mg/mL binding capacity (human IgA)
conjugate   agarose conjugate
storage temp.   2-8°C

Description

Application

Anti-Human-IgA( α-chain specific) can detect human IgA in normal and pathological situations such as cancer or autoimmune diseases. It could also be used in quantitative ELISA.

Affinity chromatography using anti-human IgA antibody linked to agarose was performed to purify proteins from 2E9IgA1 transfectant cells.

Immunogen

IgA produced from transformed cell line of human Epstein-Barr virus.

Physical form

Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

General description

IgA antibody plays an essential role in mucosal immunity; it has shown to bind immunogens/pathogens to restrict them from entering the mucosal membrane. The Anti-Human IgA (alpha-chain specific) product increases sensitivity to human IgA without cross-reactivity to other substances. Offering minimal interspecies cross reactivity to mouse or a rat, making it ideal for use in screening antibodies produced by a hybridoma cells containing a mouse or a rat immunoglobulins.
The antibody preparation is specific for human IgA when tested against purified human IgA, IgG, IgM, and kappa and lambda light chains. The product does not react with mouse or rat IgG as observed by Ouchterlony Double Diffusion (ODD), prior to agarose bead coupling.

Price and Availability


Detect Your Unseen Protein
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
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Peer-Reviewed Papers
15

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