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A270 Sigma

Anti-α1 Adrenergic Receptor antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories AD-AD, Alphabetical Index, Antibodies, Antibodies for Neurotransmission, Antibodies to Neurotransmitter Receptors,
species reactivity   rat, human, mouse
application(s)   indirect immunofluorescence: 1:1,000 using mouse kidney distal tubule cells
  western blot: 1:400
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt ~60 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... ADRA1A(148), ADRA1B(147), ADRA1D(146)
mouse ... Adra1d(11550)
rat ... Adra1d(29413)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

A270 is produced by immunizing New Zealand white rabbits with a synthetic peptide corresponding to aa residues 339-349 of human alpha 1a adrenergic receptor. This sequence is 100% conserved within the 3rd intracellular loop of all alpha1 adrenergic receptors. Peptide sequence: K339-F-S-R-E-K-K-A-A-K-T349

General description

Adrenergic receptors (ARs) are members of the 7-transmembrane domain G protein-coupled receptor superfamily that bind the endogenous catecholamines epinephrine and norepinephrine. Pharmacological, structural, and molecular cloning data indicate significant heterogeneity within this receptor family. Nine receptor subtypes have been identified thus far including three α1AR subtypes (α1A/D, α1B, and α1C), three α2ARs (α2A, α2B, and α2C), and three βAR subtypes (β1, β2, and β3). Adrenergic receptors participate in either the onset or maintenance of several disease states including hypertension, cardiac dysfunction (congestive heart failure, ischemia, arrhythmias), diabetes, glaucoma, depression, and impotence.

Adrenergic receptors are a G-protien coupled receptors that exist in two forms, α or β. Ligands for adrenergic receptors are catecholamines that induce a sympathetic response in animal cells.

Rabbit polyclonal Anti-α1 Adrenergic Receptor antibody is human α1 adrenergic receptor subtype-specific and cross-reacts with rat and mouse α1 adrenergic receptor in immunoblotting. It detects α1 adrenergic receptor in mouse kidney membrane preparations by immunoblotting and stains plasma membrane of mouse kidney distal tubule cells in immunofluorescence.

Physical form

Solution in phosphate buffered saline containing 1 mg/ml BSA and 0.05% sodium azide.

Application

Rabbit polyclonal Anti-α1 Adrenergic Receptor antibody is used for the localization and detection of the α1 adrenergic receptor (~ 60 kDa). It can be used in immunoblotting and immunofluorescence techniques.

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
Protocols & Articles

Articles

Antihypertensive Agents

Download BioFiles v7 n5 (3.18 Mb PDF) Back to Pharmaceutical Drugs and Drug Candidates homepage
Sami Barghshoon
BioFiles v7 n5, 2012, 5–20
Keywords: AGE, Antihypertensives, Cardiovascular, Clinical, Diuretics, Pharmaceutical, Reductions

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