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  • A274 - Monoclonal Anti-H+/K+ ATPase (β Subunit) antibody produced in mouse

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A274 Sigma

Monoclonal Anti-H+/K+ ATPase (β Subunit) antibody produced in mouse

clone 2G11, ascites fluid, buffered aqueous solution

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Properties

Related Categories ATH-AZ, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Ion Channels,
species reactivity   rabbit, ferret, rat, bovine, mouse, canine, pig
application(s)   immunohistochemistry (frozen sections): suitable
  indirect immunofluorescence: 1:2,000
  western blot (chemiluminescent): 1:4,000
clone   2G11, monoclonal
antibody form   ascites fluid
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 60-80 kDa (glycosylated form)
shipped in   dry ice
storage temp.   −20°C
Gene Information   mouse ... Atp4b(11945)
rat ... Atp4b(24217)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

core 34 kDa H+/K+ ATPase β subunit from deglycosylated pig gastric microsomes.

General description

The H+/K+-ATPase is a gastric proton pump that is a member of the P-type cation-transporting adenosine 5′-triphosphatase (ATPase) family. H+/K+-ATPase consists of a large transmembrane, catalytic α subunit, that contains sites for ATP binding and phosphorylation. This ATPase also has a smaller glycoprotein (β) subunit which may play a role in maintaining the structural and functional integrity of the complex. Mouse monoclonal anti-H+/K+ ATPase (β subunit) binds to H+/K+-ATPase β subunit in cow, dog, pig, rabbit, mouse, ferret and rat tissues. By immunoblotting, the antibody detects various forms of the β subunit, including a 60-80kDa glycosylated protein, a 52kDa β subunit precursor and the 34kDa core peptide.

The antibody reacts with various forms of the H+/K+ ATPase β subunit. It binds an epitope within amino acids 1-13 or 15-28 located on the cytoplasmic side of the β subunit. The antibody has been shown to inhibit the enzymatic activity of the H+/K+-ATPase and to alter the affinity of the cytoplasmic K+ binding site. It may be used to localize and detect H+/K+ ATPase β subunit.

Physical form

Solution in phosphate buffered saline containing 0.05% sodium azide.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Mouse monoclonal anti-H+/K+ ATPase (β subunit) antibody can be used for indirect immunofluorescence (1:2,000), chemiluminescent western blot (1:4,000), and immunohistochemical applications.

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
Protocols & Articles

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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