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  • A276 - Monoclonal Anti-Na+/K+ ATPase (α Subunit) antibody produced in mouse

A276 Sigma

Monoclonal Anti-Na+/K+ ATPase (α Subunit) antibody produced in mouse

clone M7-PB-E9, ascites fluid



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Ion Channels, Antibodies to Ion Pumps,
species reactivity   canine, sheep, bovine, chicken, pig, mouse, human
does not react with   rat, Xenopus
application(s)   immunocytochemistry: suitable
  immunofluorescence: 1:20
  immunohistochemistry (frozen sections): 1:100
  immunoprecipitation: suitable
  indirect ELISA: suitable
  western blot: 1:250
clone   M7-PB-E9, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt ~110 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... ATP1A1(476)
biological source   mouse
conjugate   unconjugated



sheep kidney α subunit of Na+/K+ ATPase.

General description

Epitope mapping studies indicate the epitope recognized by this clone is between amino acids 646 and 652 of the sheep kidney α subunit of Na+/K+ ATPase. The antibody does not react with rat or Xenopus α1 subunit. Staining is consistent with the plasma membrane localization of Na+/K+ ATPase.

Mouse monoclonal anti-Na+/K+ ATPase (α subunit) antibody reacts with the α1 subunit in human, mouse, bovine, sheep, canine, chicken, and pig tissues. In rats, the antibody reacts with the α3 subunit, but not with the α2 subunit.

Antigen Background

Na+/K+ transporting ATPase subunit α1 is an ion transport pump critical for maintaining the gradient of Na and K ions across the plasma membrane.

Physical form

solution containing 1 mg/ml BSA and 0.05% sodium azide


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Mouse monoclonal anti-Na+/K+ ATPase (α subunit) antibody has been used for immunoblotting assays. The product can also be used for immunofluorescence (1:20), immunohistochemistry (at 1:100 using frozen sections), immunoprecipitation, indirect ELISA and immunocytochemistry applications.

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers


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