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  • A278 - Monoclonal Anti-Na+/K+ ATPase (β1 Subunit) antibody produced in mouse

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A278 Sigma

Monoclonal Anti-Na+/K+ ATPase (β1 Subunit) antibody produced in mouse

clone M17-P5-F11

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Ion Channels, Antibodies to Ion Pumps,
species reactivity   sheep, canine, human, pig
does not react with   rat
application(s)   immunohistochemistry (frozen sections): 1:100
  western blot: 1:1,000-1:10,000
clone   M17-P5-F11, monoclonal
isotype   IgG2a
mol wt   antigen (kidney) mol wt 55 kDa
  antigen (liver) mol wt 42 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... ATP1B1(481)
conjugate   unconjugated

Description

Immunogen

lamb kidney β subunit of Na+/K+ ATPase.

General description

Epitope mapping studies indicate that this clone recognizes the epitope between amino acids 195 and 199 of the sheep β1 subunit of Na+/K+ ATPase. Staining is consistent with the plasma membrane localization of Na+/K+ ATPase.

Mouse monoclonal anti-Na+/K+ ATPase (β1 subunit) reacts with the β1 subunit of Na+/K+ ATPase in humans, sheep, canines and pigs, but not in rats. The product shows no reactivity with rat β2 subunit. In immunoblotting, the antibody detects the β1 subunit of Na+/K+ ATPase as a 42kDa band in liver samples, and as a 52kDa band in kidney samples.

Na+/K+ transporting ATPase subunit α1 is an ion transport pump critical for maintaining the gradient of Na and K ions across the plasma membrane.

Physical form

Solution in phosphate buffered saline with 1mg/ml BSA and 0.05% sodium azide

Application

Mouse Monoclonal Anti-Na+/K+ ATPase (β1 subunit) can be used in immunohistochemical (1:100) and western blot (1:1,000 - 1:10,000) applications

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