|Related Categories||1.4.x.x Acting on CH-NH2, 1.x.x.x Oxidoreductases, Amino Acid Metabolism, Biochemicals and Reagents, Cell Biology,|
|form||ammonium sulfate suspension|
Package size based on protein content
One unit will oxidatively deaminate 1.0 μmole of
Suspension in 3.2 M (NH4)2SO4 pH approx. 6
L-amino acid oxidase (LAAO) is used to convert L-amino acids to their corresponding α-keto acids. Product A2805 is from Crotalus durissus venom. L-amino acid oxidase, from Sigma, has been used in leucine aminopeptidase (LAP) activity assays 1.
L-amino acid oxidase is involved in various metabolic pathways such as alanine and aspartate metabolism, methionine metabolism, valine, leucine and isoleucine degradation, tyrosine metabolism, phenylalanine metabolism, tryptophan metabolism, phenylalanine, tyrosine and tryptophan biosynthesis, and alkaloid biosynthesis 2. It is found in microorganisms and in animal tissue, especially in kidney and liver.
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Type I, dried venom, ≥0.3 unit/mg solid
Type IV, ≥3.0 units/mg protein (biuret), aqueous suspension
Type VI, dried venom, ≥0.10 unit/mg solid
Certificate of Analysis
|Precautionary statements||P260-P264-P280-P284-P302 + P350-P310|
|Personal Protective Equipment||Eyeshields, Faceshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges|
|Hazard Codes (Europe)||T+|
|Risk Statements (Europe)||26/27/28|
|Safety Statements (Europe)||36/37/39-45|
|RIDADR||UN 3462 6.1 / PGI|
Note that for an ammonium sulfate suspension, most of the enzyme will be in solid form. It is likely that only negligible amounts of enzyme will be in the ammonium sulfate solution.
Keywords: Enzyme activity
From our library of Related Content, Sigma-Aldrich presents Enzyme Explorer: the most comprehensive source of enzymes, substrates, activators, & inhibitors.
Keywords: Cell culture, Cell disruption, Cell signaling, Diagnostic, Digestions, Drug discovery, Functional genomics, Gene expression, Genomics, Metabolic Pathways, Molecular biology, Neuroscience, Proteomics
1. Modulation of Streptomyces leucine aminopeptidase by calcium: identification and functional analysis of key residues in activation and stabilization by calcium. Jiro Arima, Yoshiko Uesugi, et al. J. Biol. Chem. 281, 5885-5894, (2006)
Cloning of a novel L-amino acid oxidase from Trichoderma harzianum ETS 323 and bioactivity analysis of overexpressed L-amino acid oxidase. Cheng CH, Yang CA, Liu SY, et al. J. Agric. Food Chem. 59(17), 9142-9, (2011)
High resolution X-ray crystal structures of L-phenylalanine oxidase (deaminating and decarboxylating) from Pseudomonas sp. P-501. Structures of the enzyme-ligand complex and catalytic mechanism. Ida K, Suguro M, and Suzuki H J. Biochem. 150(6), 659-69, (2011)
Proteomic and biological characterization of the venom of the redtail coral snake, Micrurus mipartitus (Elapidae), from Colombia and Costa Rica. Rey-Suárez P, Núñez V, Gutiérrez JM, et al. J. Proteomics 75(2), 655-67, (2011)
Novel L-amino acid oxidase with algicidal activity against toxic cyanobacterium Microcystis aeruginosa synthesized by a bacterium Aquimarina sp. Chen WM, Sheu FS, and Sheu SY Enzyme Microb. Technol. 49(4), 372-9, (2011)
Enzymatic preparation of 5-hydroxy-L-proline, N-Cbz-5-hydroxy-L-proline, and N-Boc-5-hydroxy-L-proline from (α-N-protected)-L-ornithine using a transaminase or an amine oxidase. Hanson RL, Johnston RM, Goldberg SL, et al. Enzyme Microb. Technol. 48(6-7), 445-53, (2011)
Mechanisms of action of escapin, a bactericidal agent in the ink secretion of the sea hare Aplysia californica: rapid and long-lasting DNA condensation and involvement of the OxyR-regulated oxidative stress pathway. Ko KC, Tai PC, and Derby CD Antimicrob. Agents Chemother. 56(4), 1725-34, (2012)
Crystallization and preliminary X-ray diffraction analysis of an L-amino-acid oxidase from Bothrops jararacussu venom. Ullah A, Coronado M, Murakami MT, et al. Acta Crystallogr. F, Struct. Biol. Cryst. Commun. 68(Pt 2), 211-3, (2012)
Monomeric L-amino acid oxidase-induced mitochondrial dysfunction in Rhizoctonia solani Reveals a novel antagonistic mechanism of Trichoderma harzianum ETS 323. Yang CA, Cheng CH, Lee JW, et al. J. Agric. Food Chem. 60(10), 2464-71, (2012)
Positively charged mini-protein Zbasic2 as a highly efficient silica binding module: opportunities for enzyme immobilization on unmodified silica supports. Bolivar JM and Nidetzky B Langmuir 28(26), 10040-9, (2012)
Analysis of phospholipase A2, L-amino acid oxidase, and proteinase enzymatic activities of the Lachesis muta rhombeata venom. Campos LB, Pucca MB, Roncolato EC, et al. J. Biochem. Mol. Toxicol. 26(8), 308-14, (2012)
Isolation and biochemical, functional and structural characterization of a novel L-amino acid oxidase from Lachesis muta snake venom. Bregge-Silva C, Nonato MC, de Albuquerque S, et al. Toxicon 60(7), 1263-76, (2012)
The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. Clark HF, Gurney AL, Abaya E, et al. Genome Res. 13(10), 2265-70, (2003)
The Journal of Venomous Animals and Toxins including Tropical Diseases: "Antimicrobial activity of an L-amino acid oxidase isolated from Bothrops leucurus snake venom" Torres AFC, et al. J. Affect. Disord. 16, 614-622, (2010)
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