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A2831 Sigma

Monoclonal Anti-DNP−Alkaline Phosphatase Conjugate antibody produced in mouse

clone SPE-7, purified immunoglobulin, buffered aqueous glycerol solution

Synonym: Anti-Dinitrophenyl, Monoclonal Anti-DNP

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies to Dinitrophenyl Group (DNP), DM-DP, Molecular Biology,
biological source   mouse
antibody form   purified immunoglobulin
clone   SPE-7, monoclonal
form   buffered aqueous glycerol solution
application(s)   direct ELISA: 1:25,000 using DNP-BSA
  dot blot: 1:180,000
isotype   IgE
conjugate   alkaline phosphatase conjugate
shipped in   wet ice
storage temp.   2-8°C

Description

Analysis Note

Monoclonal Anti-DNP is tested to ensure minimal reactivity against bovine serum albumin.

Immunogen

DNP-KLH conjugate

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% BSA, and 50% glycerol and 15 mM Sodium azide

Application

Mouse monoclonal anti-DNP-alkaline phosphatase conjugate antibody can be used for ELISA (1:25,000) and dot blot (1:180,000) assays.

The antibody may be used for experimental induction of controlled allergic reactions or as a source of mouse IgE.

The antibody may be used for experimental induction of controlled allergic reactions or as a source of mouse IgE.To induce systemic anaphylaxis, mice were injected with 3 μg of anti-DNP mouse IgE and then given by IV 500 μg of DNP-BSA twenty hours later. To induce local anaphylaxis, mice were injected intradermally with 10ng of anti-DNP mouse IgE and then challenged with an i.v. injection of 500 μg DNP-BSA.

General description

Dinitrophenyl (DNP) is a hapten that is used for labeling primary or secondary probes in immunological analyses. Thus, anti-DNP antibody conjugated to a detectable substrate is a useful tool for the study of target proteins. Mouse monoclonal anti-DNP-alkaline phosphatase conjugate antibody is specific for DNP.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
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Peer-Reviewed Papers
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