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A2856 Sigma

Anti-ATG7 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-Apoptosis-specific-protein, Anti-APG7-like, Anti-APG7, Anti-APG7L, Anti-Autophagy protein 7-like, Anti-Ubiquitin activating enzyme E1-like protein

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Properties

Related Categories AS-ATG, Alphabetical Index, Antibodies, Antibodies for Autophagy, Antibodies for Cell Biology,
species reactivity   human, mouse, rat
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2.5-5 μg/mL using biotin/ExtraAvidin®-Peroxidase staining of heat-retrieved sections of human brain
  immunoprecipitation: 1-2 μL using human U87 cell lysate
  western blot: 0.5-1 μg/mL using whole extracts of rat PC12 and mouse 3T3 cells
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt ~75 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... ATG7(10533)
mouse ... Atg7(74244)
rat ... Atg7(312647)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 38-50 of human ATG7, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in mouse and rat.

General description

Autophagy is a catabolic process by which cell delivers cytoplasmic constituent into lysosome for protein degradation. 16 ATG genes are essential for autophagosome preparation out of which Atg7 is the one required to activate ubiquitin-like proteins Atg12 and Atg8. Atg7 is also crucial for amino acid supply in neonates, as well as for bulk degradation of proteins and organelles in mice during starvation. Anti-ATG7 antibody can be used in immunohistochemistry and immunoprecipitation. Rabbit anti-ATG7 antibody reacts specifically with Atg7 of human, mouse and rat.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-ATG7 antibody can be used in western blotting and immunoblotting.

Rabbit anti-ATG7 antibody has been used for immunoblotting assays. The antibody is also suitable for use in immunoprecipitation applications at 1-2μL using human U87 cell lysates.

Rabbit anti-ATG7 antibody was used for immunohistochemistry on sections of mouse midbrain fixed in 4% paraformaldehyde at 1:2000.

Legal Information

ExtrAvidin is a registered trademark of Sigma-Aldrich Co. LLC

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Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  3
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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
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