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A2901 Sigma

Avidin–FITC from egg white

lyophilized powder

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Properties

Related Categories Antibodies, Avidin Reagents, Biotin/Avidin, Supplementary Products
form   lyophilized powder
extent of labeling   2-4 mol fluorescein isothiocyanate per mol avidin
application(s)   immunohistochemistry: suitable
conjugate   FITC conjugate
storage temp.   −20°C

Description

Packaging

Package size based on protein content

Physical form

Contains sodium citrate

Application

Avidin-FITC from egg white has been used for immunohistochemistry applications.

To determine the amount of labeled polyclonal IgG present in spores in an IgG prep. A known concentration of FITC avidin from egg white and biotin-IgG avidin was used in excess at 300nm.

General description

Avidin is homotetrameric protein (68kDa) that is obtained from egg whites. This egg protein binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

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Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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