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A2909 Sigma

Rabbit IgG−Agarose

saline suspension

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Properties

Related Categories Adsorption Resins, Antibodies, Antibody Purification and Characterization, Supplementary Products
form   saline suspension
extent of labeling   ≥5 mg per mL
application(s)   immunoprecipitation: suitable
matrix   cross-linked 4% beaded agarose
matrix activation   cyanogen bromide
matrix spacer   1 atom
conjugate   agarose conjugate
storage temp.   2-8°C

Description

Packaging

2, 5 mL in glass bottle

Physical form

suspension in 0.5 M NaCl containing 0.01% thimerosal

Application

Rabbit IgG antibody (20 μl/ml) crosslinked to agarose beads was used to isolate RAT tagged proteins from whole cell extracts of 293T cells.Rabbit IgG crosslinked to agarose beads were used for purification of tagged protein from mammalian whole cell extracts at 0.5 to 1.0mg total protein to 10 μl of beads.

Rabbit IgG-Agarose has been used for immunoprecipitation and affinity purification assays.

General description

IgGs are glycoprotein antibodies that modulate several immune responses. IgG-Agarose is an immunoadsorbent that can be used to purify antibodies, remove species specific cross-reacting antibodies, or remove contaminating antibodies from an antiserum preparation. Characteristically, cross-reacting antibodies may be removed from an antiserum preparation using an equal resin volume of IgG-Agarose. However, the resin to antiserum ratio will vary with individual applications.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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