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A2930 Sigma

Avidin–β-galactosidase Labeled from egg white

lyophilized powder, galactosidase 200-600 units/mg protein

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Properties

form   lyophilized powder
composition   Protein, ~60% E1%/280
extent of labeling   1-3 mol avidin per mol galactosidase
solubility   H2O: soluble1 mg/mL, clear, colorless
storage temp.   −20°C

Description

Packaging

Package size based on protein content

Preparation Note

Prepared from A9275, partially acetylated, and β-galactosidase (G5635) by the modification of Sullivan, M.J., et al., FEBS Lett., 95, 311 (1978).

Unit Definition

One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactoside per min at pH 7.3 at 37 °C.

Physical form

Lyophilized powder containing Tris-succinate with a trace of dithiothreitol.

General description

Avidin is homotetrameric protein (68kDa) that is obtained from egg whites. This egg protein binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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