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A2942 Sigma

Amphotericin B solution

250 μg/mL in deionized water, sterile-filtered, BioReagent, suitable for cell culture

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Properties

Related Categories Antibiotic Solutions, Antibiotics, Antibiotics A to Z, Antibiotics A-F, Antifungal,
InChI Key   APKFDSVGJQXUKY-INPOYWNPSA-N
sterility   sterile-filtered
product line   BioReagent
concentration   250 μg/mL in deionized water
suitability   suitable for cell culture
Mode of action   cell membrane | interferes
antibiotic activity spectrum   fungi
  yeast
shipped in   dry ice
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Components

This product is supplied as 250 μg/mL Amphotericin B in deionized water. It is a solution containing Amphotericin B, and the following components aimed to improve its solubility: sodium deoxycholate, sodium chloride and sodium phosphate.

Application

Amphotericin B is a polyene antifungal antibiotic from Streptomyce sp. It has a high affinity for sterols, especially ergosterols, of both fungal and bacterial cell membranes. Normal usage in maintenance of antifungal cell cultures is 2.5 mg/L with penicillin and streptomycin used in the medium.

Caution

Amphotericin B remains active for 3 days in culture at 37°C. At -20°C, and with protection from air and light, the product remains active for 5 years.

Preparation Note

It is not unusual for this material to form a precipitate in aqueous solutions. Mixing the solution will redissolve most of the precipitate but, if it does not fully resolubilize, the Amphotericin B will still be suitable for use.

Biochem/physiol Actions

Amphotericin B solution has been used-
• as an intraperitoneal injection for the treatment of Aspergillus fumigatus infection in mice
• as a constituent of aspiration medium for the culture of Chinese Merino ewe ovaries
• as a constituent of transport medium during the collection of equine corpora lutea
• as a supplement in RPMI medium for the culture of the mouse-mouse B cell hybridoma 55-6 cell line

General description

Amphotericin B is a polyene antifungal antibiotic that is clinically used to treat systemic fungal infections.

Protocols & Applications

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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