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A2951 Sigma

Monoclonal Anti-Alkaline Phosphatase, Human Placental antibody produced in mouse

clone 8B6, ascites fluid

Synonym: Anti-Phosphatase, Alkaline, Human Placental, Monoclonal Anti-Alkaline Phosphatase, Human Placental, PLAP

Properties

Related Categories AL-AL, Alkaline Phosphatase Detection, Alphabetical Index, Antibodies, Antibodies to Peptide and Protein Fusion Tags,
species reactivity   human
application(s)   immunocytochemistry: suitable
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:4,000 using human placenta
  immunohistochemistry (frozen sections): suitable
  indirect ELISA: suitable using whole cells
  radioimmunoassay: suitable using radio-immunolocalization of xenografts of human cell lines expressing PLAP in nude mice2
  western blot: suitable
clone   8B6, monoclonal
antibody form   ascites fluid
isotype   IgG2a
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... ALPP(250)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

Human epidermoid carcinoma cell line expressing placental alkaline phosphatase.

General description

Human placental alkaline phosphatase (EC 3.1.3.1; hPLAP) is an isoenzyme that is usually synthesized in the placental syncytiotrophoblast and becomes detectable in the maternal circulation after the twelfth week of pregnancy. Although hPLAP has been analyzed as a potential tumor marker, it has not been widely used in the routine clinical laboratory because of its low overall specificity due to heat-inactivation, cross-reactivity with intestinal AP and L-phenylalanine sensitivity. The use of monoclonal antibodies as reagents has greatly improved the sensitivity, reproducibility and accuracy of the tests used to distinguish placental isozymes from their tumor cell counterparts. Thus, monoclonal anti-alkaline phosphatase may be useful as diagnostic tools for tumor detection.

In SDS gels, the product reacts with both Regan and Nagao isozymes of human placental alkaline phosphatase (hPLAP, 130 kDa, 67/130 kDa). By RIA, the antibody binds to hPLAP with an affinity constant of 5 × 109 LM-1. It does not react with PLAP-like enzymes.

Application

Anti-Alkaline phosphatase human placental antibody coupled to agarose was packed in a column and used to purify alkaline-phosphatase conjugated FGF receptor out of conditioned media from COS-7 cells. Column was washed with 1M NaCl.

Cultured pancreatic islet cells were fixed with cold 4% paraformaldhyde at room temperature for 10 minutes and incubated with mouse monoclonal anti-Alkaline Phosphatase, Human Placental antibody at 1:500. Mouse monoclonal anti-Alkaline Phosphatase, Human Placental antibody was used to detect a unique molecular probe that consisted of the FGFR extracellular domain expressed as a chimera with human PLAP.

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