Monoclonal Anti-Alkaline Phosphatase, Human Placental antibody produced in mouse
clone 8B6, ascites fluid
DOWNLOAD MSDS (PDF)Synonym: Anti-Phosphatase, Alkaline, Human Placental, Monoclonal Anti-Alkaline Phosphatase, Human Placental, PLAP
-
MDL number MFCD00162106
-
Popular Documents: Datasheet (PDF)
Properties
| Related Categories | 8, AL-AL, Alkaline Phosphatase Detection, Alphabetical Index, Antibodies, |
| species reactivity | human |
| application(s) | immunocytochemistry: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:4,000 using human placenta | |
| immunohistochemistry (frozen sections): suitable | |
| indirect ELISA: suitable using whole cells | |
| radioimmunoassay: suitable using radio-immunolocalization of xenografts of human cell lines expressing PLAP in nude mice2 | |
| western blot: suitable | |
| clone | 8B6, monoclonal |
| antibody form | ascites fluid |
| isotype | IgG2a |
| contains | 15 mM sodium azide |
| shipped in | dry ice |
| storage temp. | −20°C |
| Gene Information | human ... ALPP(250) |
Description
Immunogen
Human epidermoid carcinoma cell line expressing placental alkaline phosphatase.
Specificity
In SDS gels, the product reacts with both Regan and Nagao isozymes of human placental alkaline phosphatase (hPLAP, 130 kDa, 67/130 kDa). By RIA, the antibody binds to hPLAP with an affinity constant of 5 × 109 LM-1. It does not react with PLAP-like enzymes.
Application
Anti-Alkaline phosphatase human placental antibody coupled to agarose was packed in a column and used to purify alkaline-phosphatase conjugated FGF receptor out of conditioned media from COS-7 cells. Column was washed with 1M NaCl.1
Cultured pancreatic islet cells were fixed with cold 4% paraformaldhyde at room temperature for 10 minutes and incubated with mouse monoclonal anti-Alkaline Phosphatase, Human Placental antibody at 1:500. Mouse monoclonal anti-Alkaline Phosphatase, Human Placental antibody was used to detect a unique molecular probe that consisted of the FGFR extracellular domain expressed as a chimera with human PLAP.
Price and Availability
Customers Also Viewed
clone AP1B9, purified immunoglobulin, buffered aqueous solution
affinity isolated antibody, buffered aqueous solution
clone 8B6, purified immunoglobulin, PBS suspension
≥10 units/mg solid (in glycine buffer)
Technical Service:
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Bulk Ordering & Pricing:
Need larger quantities for your development, manufacturing or research applications?
