Synonym: Anti-Phosphatase, Alkaline, Human Placental, Monoclonal Anti-Alkaline Phosphatase, Human Placental, PLAP
|Related Categories||8, AL-AL, Alkaline Phosphatase Detection, Alphabetical Index, Antibodies,|
|immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:4,000 using human placenta|
|immunohistochemistry (frozen sections): suitable|
|indirect ELISA: suitable using whole cells|
|radioimmunoassay: suitable using radio-immunolocalization of xenografts of human cell lines expressing PLAP in nude mice2|
|western blot: suitable|
|antibody form||ascites fluid|
|contains||15 mM sodium azide|
|shipped in||dry ice|
|Gene Information||human ... ALPP(250)|
Human epidermoid carcinoma cell line expressing placental alkaline phosphatase.
In SDS gels, the product reacts with both Regan and Nagao isozymes of human placental alkaline phosphatase (hPLAP, 130 kDa, 67/130 kDa). By RIA, the antibody binds to hPLAP with an affinity constant of 5 × 109 LM-1. It does not react with PLAP-like enzymes.
Anti-Alkaline phosphatase human placental antibody coupled to agarose was packed in a column and used to purify alkaline-phosphatase conjugated FGF receptor out of conditioned media from COS-7 cells. Column was washed with 1M NaCl.1
Cultured pancreatic islet cells were fixed with cold 4% paraformaldhyde at room temperature for 10 minutes and incubated with mouse monoclonal anti-Alkaline Phosphatase, Human Placental antibody at 1:500. Mouse monoclonal anti-Alkaline Phosphatase, Human Placental antibody was used to detect a unique molecular probe that consisted of the FGFR extracellular domain expressed as a chimera with human PLAP.
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Keywords: Buffers, Cell biology, Epigenetics, Immunohistochemistry, Immunoprecipitation, Molecular biology, Neuroscience, Western blot
Intrinsic differences among spatially distinct neural crest stem cells in terms of migratory properties, fate-determination, and ability to colonize the enteric nervous system Dlugosz, A.A., et al. Dev. Biol. 303, 1-15, (2007)
Production of monoclonal antibodies to placental alkaline phosphatase: preliminary characterisation includes identification of one antibody reactive with routinely fixed histological preparations. Durbin, H., et al. Int. J. Cancer Suppl., 2, 50, (1988)
Monoclonal antibodies to placental alkaline phosphatase: preclinical evaluation in a human xenograft tumour model of F(ab')2 and Fab fragments. Durbin, H., et al. Int. J. Cancer Suppl., 2, 59, (1988)
Highly Productive Infection with Pseudotyped Human Immunodeficiency Virus Type 1 (HIV-1) Indicates No Intracellular Restrictions to HIV-1 Replication in Primary Human Astrocytes Canki, M. et. al. J. Virol. 75, 7925-7933, (2001)
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