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A2981 Sigma

Anti-ATG4B antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: ATG4, Anti-Autophagy-related cysteine endopeptidase 1, Anti-Autophagy-related protein 4 homolog B, Anti-APG4B, Anti-AUT-like 1 cysteine endopeptidase, Anti-AUTL1, Anti-Autophagin-1, Anti-Cysteine protease ATG4B, Anti-KIAA0943, Anti-hAPG4B

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Properties

Related Categories AS-ATG, Alphabetical Index, Antibodies, Antibodies for Autophagy, Antibodies for Cell Biology,
species reactivity   rat, human, mouse
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 using biotin/ExtaAvidin®-Peroxidase staining of heat-retrieved human cerebellum sections
  western blot: 1:125-1:500 using whole extract of 3T3 cells
  western blot: 1:500-1:1,000 using whole extract of rat brain
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt ~44 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... ATG4B(23192)
mouse ... Atg4b(66615)
rat ... Atg4b(316640)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 6-22 of human ATG4B, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in mouse.

General description

ATG4B is a member of the C-54 family of cysteine proteases that is involved in cell degradation through autophagy.

Rabbit anti-ATG4B antibody binds to human, mouse and rat ATGB4 by immunoblotting (approx. 44kDa) and immunohistochemistry. Detection of the ATGB4 band is specifically inhibited by the immunizing peptide in immunoblotting assays.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)

Rabbit anti-ATG4B antibody has been used for immunoblotting applications. The antibody can also be used for immunohistochemical assays using formalin-fixed, paraffin-embedded sections at dilutions ranging from 1:50-1:100.

Legal Information

ExtrAvidin is a registered trademark of Sigma-Aldrich Co. LLC

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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SAB2100172 Anti-ATG4B antibody produced in rabbit, affinity isolated antibody

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