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A3059 Sigma

Bovine Serum Albumin

heat shock fraction, protease free, essentially globulin free, pH 7, ≥98%

Synonym: Albumin bovine serum, BSA, Bovine albumin

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Properties

Related Categories Albumin, Biochemicals and Reagents, Bovine Serum Albumins, Core Bioreagents, General Reagents,
assay   ≥98%
form   lyophilized powder
mol wt   mol wt ~66 kDa
purified by   heat shock fractionation
packaging   poly bottle of
origin   USA origin
impurities   essentially globulin free
loss   ≤5%
pH   7
solubility   water: soluble (40 mg/ml)
foreign activity   protease ≤0.005 U/mg
storage temp.   2-8°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Packaging

10, 50, 100, 500 g in poly bottle

Preparation Note

Prepared using heat shock fractionation

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

Is Product A3059, Albumin from bovine serum (BSA), suitable for use in ELISA?
We have not tested A3059 for specific use in ELISA. However, A3059 is considered to be essentially gamma-globulin free, which makes this more potentially useful in ELISA, by reducing a potential source of background.
Is Product A3059, Albumin from bovine serum (BSA), suitable for use as a blocking agent in immunoblotting?
We have not tested A3059 for specific use in immunoblotting as a blocking agent, in quality control. However, A3059 is considered to be essentially gamma-globulin free, which makes this more potentially useful in blotting experiments, by reducing a potential source of background.  The Technical Bulletin states that "Globulin free albumins are suitable for use in applications where no other proteins should be present". To access the Technical Bulletin on A3059, click here:  http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/a3059pis.Par.0001.File.tmp/a3059pis.pdf
What is the fatty acid content of Product A3059, Albumin from bovine serum (BSA)?  Can it be considered fatty acid free?
The fatty acid content of A3059 is not tested in quality control, and it is not reported on our Certificate of Analysis. We would not refer to this product as "fatty acid free".
Is Product A3059, Albumin from bovine serum (BSA), endotoxin-free?
We have not tested A3059 for endotoxin content. Endotoxin-tested BSA products would include A4919, A9306, A9430, A2934, A9306, A9543, or A3675.
Is Product A3059, Albumin from bovine serum (BSA), acetylated to inactivate nucleases?  
A3059 is not acetylated. We do not test for the presence of nucleases. Acetylated BSA products would be B2518 or B8894.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
I need help selecting an Albumin from bovine serum (BSA) product, is there a guide to help me choose the best product?
Yes,  a selection guide to our BSA products can be found at the link below: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/General_Information/albumin_application_selection_guide1.Par.0001.File.tmp/albumin_application_selection_guide1.pdf
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Protocols & Articles
Peer-Reviewed Papers
15

References

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