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A3151 Sigma

Avidin–Peroxidase

lyophilized powder

Synonym: Avidin-POD

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Properties

Related Categories Antibodies, Antibody Conjugates, Avidin Reagents, Biotin/Avidin, Molecular Biology,
biological source   Avidin from egg white
  Peroxidase from horseradish
form   lyophilized powder
composition   Protein, ≥70% E1%/280
extent of labeling   0.7-2.0 mol peroxidase per mol avidin
application(s)   direct ELISA: 1:50,000
solubility   H2O: soluble 1 mg/mL
  PBS: soluble
conjugate   peroxidase conjugate
storage temp.   −20°C

Description

Preparation Note

Labeled with Type VI peroxidase. Coupled by a modification of the method of Sullivan, M.J., et al., FEBS Lett., 95, 311 (1978).

Physical form

Lyophilized powder containing citrate buffer.

Application

Avidin-Peroxidase has been used for detecting anti-5-bromodeoxyuridine (anti-BrdU) binding and OVA-IgG1.

To detect Ga1-deficient IgA1 antibodies in mouse serum, ELISA assays were performed using sera which was incubated with biotinylated HAA, which binds IgA1, in 96-well plates. HRP-conjugated avidin was then incubated with the sera for 40 minutes at 37 degrees.

General description

Avidin is homotetrameric protein (68kDa) that is obtained from egg whites. This egg protein binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.

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RIDADR 
NONH for all modes of transport
WGK Germany 
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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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