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A3156 Sigma

Bovine Serum Albumin

lyophilized powder, γ-irradiated, Globulin Free, BioXtra, suitable for cell culture

Synonym: Albumin bovine serum, BSA, Bovine albumin

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Description

Preparation Note

Purified by a combination of heat shock and ethanol fractionation

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Application

Qualified for use in cell culture applications.

Biochem/physiol Actions

Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media. It provides a range of benefits including protection from oxidative damage and stabilization of other media components such as fatty acids and pyridoxal.

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

General description

BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7 it contains 17 intrachain disulfide bridges and 1 sulfhydryl group.

Protocols & Applications

Select the right albumin for your application with the Albumin Selection Guide

Price and Availability


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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles
Peer-Reviewed Papers
15

References

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