Anti-Human IgG (γ-chain specific)−Alkaline Phosphatase antibody produced in goat

Other Notes

Antibody adsorbed with mouse serum proteins.

Immunogen

Purified human IgG.

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl₂, 10 mM glycine, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide as preservative

Application

Anti-human IgG (γ-chain specific) −alkaline phosphatase antibody can be used in immunocytochemical staining techniques¹. It can also be used for dot blot.

IgG was detected in human serum by ELISA using alkaline phosphatase conjugated goat anti-human IgG (γ-chain specific) diluted 1:5000 in 0.25% BSA/PBS. Assay was developed using p-nitrophenyl phosphate substrate (1mg/ml Sigma).

Proteins harvested from E. coli cultures were run on SDS-page gel for western blot analysis using alkaline phosphatase conjugated goat anti-human IgG (chain specific) at a concentration of 1:10000 for the secondary. IgG was detected in human serum by Elisa using alkaline phosphatase conjugated goat anti-human IgG (gamma-chain specific) diluted 1:5000 in 0.25% BSA/PBS. Assay was developed using p-nitrophenyl phosphate substrate (1mg/ml Sigma).

General description

Human IgG antibodies are primarily responsible for the facilitation of humoral immune responses such as complement fixation, placental transport, and phagocytosis². Anti-human IgG (γ-chain specific) −alkaline phosphatase antibody can be used in immunohistochemistry and immunoelectrophoresis. It can also be used in western blotting. Goat anti-human IgG−alkaline phosphatase antibody reacts specifically with human IgG when tested against human IgA, IgG, IgM, Bence Jones κ, and λ myeloma proteins. The product is not reactive with mouse IgG or mouse serum proteins.