|Related Categories||Alkaline Phosphatase, Alphabetical Index, Antibodies, HU-HZ, Human IgG Secondary Antibodies and Conjugates,|
|antibody form||affinity isolated antibody|
|form||buffered aqueous glycerol solution|
|does not react with||mouse|
|application(s)||direct ELISA: 1:30,000|
|immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50|
|western blot: 1:30,000|
|shipped in||wet ice|
Antibody adsorbed with mouse serum proteins.
Purified human IgG.
Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 10 mM glycine, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide as preservative
Anti-human IgG (γ-chain specific) −alkaline phosphatase antibody can be used in immunocytochemical staining techniques1. It can also be used for dot blot.
Proteins harvested from E. coli cultures were run on SDS-page gel for western blot analysis using alkaline phosphatase conjugated goat anti-human IgG (chain specific) at a concentration of 1:10000 for the secondary. IgG was detected in human serum by Elisa using alkaline phosphatase conjugated goat anti-human IgG (gamma-chain specific) diluted 1:5000 in 0.25% BSA/PBS. Assay was developed using p-nitrophenyl phosphate substrate (1mg/ml Sigma).
Human IgG antibodies are primarily responsible for the facilitation of humoral immune responses such as complement fixation, placental transport, and phagocytosis2. Anti-human IgG (γ-chain specific) −alkaline phosphatase antibody can be used in immunohistochemistry and immunoelectrophoresis. It can also be used in western blotting. Goat anti-human IgG−alkaline phosphatase antibody reacts specifically with human IgG when tested against human IgA, IgG, IgM, Bence Jones κ, and λ myeloma proteins. The product is not reactive with mouse IgG or mouse serum proteins.
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Keywords: Buffers, Cell biology, Epigenetics, Immunohistochemistry, Immunoprecipitation, Molecular biology, Neuroscience, Western blot
1. Cranial bone defect healing is accelerated by mesenchymal stem cells induced by coadministration of bone morphogenetic protein-2 and basic fibroblast growth factor. Sadanori Akita Wound Repair Regen. 12(2), 252-9, (2004)
Salivary anti-capsular antibodies in infants and children immunised with Streptococcus pneumoniae capsular polysaccharides conjugated to diphtheria or tetanus toxoid. Ahman, H., et al. Vaccine 18, 1218-1226, (2000)
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