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A3311 Sigma

Bovine Serum Albumin

powder, BioXtra

Synonym: Albumin bovine serum, BSA, Bovine albumin

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Properties

Related Categories Albumin, Albumins by Application, Biochemicals and Reagents, Bovine Serum Albumins, Cell Culture,
product line   BioXtra
assay   ≥98% (agarose gel electrophoresis)
form   powder
mol wt   mol wt ~66 kDa
packaging   poly bottle of
loss   ≤5%
pH   6.5-7.5
solubility   water: soluble (40 mg/ml)
suitability   suitable for mouse embryo cell culture
storage temp.   2-8°C

Description

Packaging

10, 50, 100 g in poly bottle

Preparation Note

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Application

This albumin has been qualified for use in media designed to support in vitro applications such as mouse blastocyst and morulae culture.

Biochem/physiol Actions

Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media. It provides a range of benefits including protection from oxidative damage and stabilization of other media components such as fatty acids and pyridoxal.

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Protocols & Applications

Select the right albumin for your application with the Albumin Selection Guide

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Related Content

Albumin in Cell Culture

Importance and uses of albumin in serum-free eucaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell cultures
Keywords: Cell culture, Culture media, Degradations, Hormones, Ligands, Oxidations, PAGE, Purification, Redox Reactions

Peer-Reviewed Papers
15

References

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