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A3428 Sigma

Aprotinin from bovine lung

lyophilized powder, 3-8 TIU/mg solid, BioReagent, suitable for cell culture

Synonym: Trasylol, Trypsin inhibitor (basic)

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Properties

Related Categories Biochemicals and Reagents, Cell Culture, Cell Dissociation, Core Bioreagents, Enzyme Inhibitors,
product line   BioReagent
form   lyophilized powder
mol wt   mol wt ~6,500
solubility   H2O: 5 mg/mL
suitability   suitable for cell culture
storage temp.   2-8°C
Gene Information   cow ... PTI(404172)

Description

Application

Aprotinin is largely used as an inhibitor of trypsin.

Packaging

10, 25 mg in poly bottle

100, 250 mg in glass bottle

Preparation Note

This product is a lyophilized powder that has been cell culture tested. It has an activity of 3-7 TIU/mg.

Unit Definition

One Trypsin Inhibitor Unit (TIU) will decrease the activity of two trypsin units by 50%, where one trypsin unit will hydrolyze 1.0 μmole of N-alpha-benzoyl-DL-arginine p-nitroanilide per minute at pH 7.8 and 25°C. Another commonly used unit is the KIU, with 1 TIU = 1,300 KIU.

When benzoyl-L-arginine ethyl ester (BAEE) is used as substrate one BAPNA unit is approx. 45BAEE μmolar units at pH8.0 at 25°C or approx. 9,000 BAEE A253 units at pH7.6 at 25°C.

Biochem/physiol Actions

Aprotinin is a competitive serine protease inhibitor that forms stable complexes with and blocks the active sites of enzyme. This binding is reversible, and most aprotinin-protease complexes will dissociate at extreme pH levels >10 or <3. Structurally, Aprotinin is a monomeric globular protein derived from bovine lung that consists of 58 amino acids, arranged in a single polypeptide chain with three crosslinking disulfide bridges.

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
YN5080000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Protocols

Enzymatic Assay of Aprotinin

The scope of this procedure is all products that have a specification for Aprotinin activity.
Keywords: Enzymology, Extinction coefficient

Peer-Reviewed Papers
15

References

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