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A3535 Sigma

α-Amylase Inhibitor from Triticum aestivum (wheat seed)

Type III, lyophilized powder

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Properties

Related Categories A to C, Amylase, alpha-, Biochemicals and Reagents, Enzyme Inhibitors, Enzyme Inhibitors by Enzyme,
type   Type III
form   lyophilized powder
activity   3000-9000 inhibitor U/mg protein (using human salivary α-amylase)
  5-100 inhibitor U/mg protein (using porcine pancreatic α-amylase)
composition   Protein, 40-60% biuret
foreign activity   α-amylase ≤1 unit/mg protein
storage temp.   −20°C

Description

Unit Definition

One unit will reduce the activity of two units of α-amylase (A0521) by 50% after pre-incubation at 25 °C.

Physical form

Lyophilized powder containing buffer salts as sodium phosphate

Biochem/physiol Actions

Competitive inhibitor of human salivary α-amylase. KI = 2.9 nM, compared to a KM of 5.9 mM (calculated per mole of α-1,4-linked maltose residues).

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Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles
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