Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody produced in goat affinity isolated antibody, buffered aqueous glycerol solution

Properties

Related Categories	Alkaline Phosphatase, Alkaline Phosphatase Conjugates, Alphabetical Index, Antibodies, Antibody Conjugates, Antibody-Alkaline Phosphatase Conjugates, MO-MR, Mouse Secondary Antibodies and Conjugates, Phosphorylation Analysis, Plant Biotechnology, Plant Immunochemistry, Post-Translational Modification, Primary Antibodies, Protein Electrophoresis, Proteomics, Reagents for Antibody Detection, Secondaries listed by Conjugate, Secondaries listed by Target Species, Secondary Antibodies, Secondary Antibodies and Labeled Streptavidin, Western Blotting More...
antibody form	affinity isolated antibody
clone	polyclonal
form	buffered aqueous glycerol solution
application(s)	direct ELISA: 1:30,000
	immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
	western blot: 1:30,000
shipped in	wet ice
storage temp.	2-8°C

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Description

Immunogen

Purified mouse IgG

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl₂, 10 mM glycine, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide.

Application

Anti-Mouse IgG (whole molecule)-Alkaline Phosphatase antibody is suitable for use in direct ELISA (1:3000¹) and western blot². The product can also be used for immunohistochemistry (1:50 using formalin-fixed, paraffin-embedded sections).

Surfactant Protein A was detected in bronchoalveolar fluid using alkaline phosphatase conjugated goat anti-mouse IgG as the secondary at μg/ml in TBS/Tween containing final concentration of 0.5M NaCl.

General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases ³. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice ⁴. Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody is specific for normal mouse serum and mouse IgG. In Ouchterlony double diffusion assays, the antibody reacts with mouse IgG1, IgG2a, IgG2b, IgG3, IgA, and IgM.

Price and Availability

Related Papers

1. Human Immunodeficiency Virus Type 1 gp41 Antibodies That Mask Membrane Proximal Region Epitopes: Antibody Binding Kinetics, Induction, and Potential for Regulation in Acute Infection Haynes, B.F., et al. J. Virol. 82, 115-125, (2008)

Documents

Certificate of Analysis

Certificate of Origin

A3562 - Datasheet (28 KB)

Antibody Explorer

Plant Biotech Guide (186 KB )

Safety Information

WGK Germany

2

Technical information & documentation associated with this product is available in the Safety & Documentation tab.

Articles

High Throughput Screening of Peptide Substrates for Tyrosine Kinases

Protein Tyrosine Kinases (PTKs) play important roles in modulating a wide variety of cellular events, including differentiation, proliferation, metabolism, and apoptosis. Therefore, identification of...
Keywords: Apoptosis, Enzyme-linked immunosorbent assay, Infrared spectroscopy, Metabolism, Peptide synthesis, Phosphorylations

High-throughput Screening of Peptide Substrates for Tyrosine Kinases Using PEPscreen® Custom Peptide Library

Protein Tyrosine Kinases (PTKs) play important roles in modulating a wide variety of cellular events, including differentiation, proliferation, metabolism and apoptosis. Those regulations are mediate...
Danhui Wang, Fan Zhang, Tao Zhao, Fei Zhong, Keming Song
Keywords: Apoptosis, Enzyme-linked immunosorbent assay, Metabolism, Peptide synthesis, Pharmaceutical, Phosphorylations

Papers

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1. Human Immunodeficiency Virus Type 1 gp41 Antibodies That Mask Membrane Proximal Region Epitopes: Antibody Binding Kinetics, Induction, and Potential for Regulation in Acute Infection Haynes, B.F., et al. J. Virol. 82, 115-125, (2008)

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2. Phosphorylation Of Profilin By ROCK1 Regulates Polyglutamine Aggregation. Shao, J., et al. Mol. Cell. Endocrinol. 28, 5196-208, (2008)

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3. Structure and Function of Human and Murine Receptors for IgG. Unkeless, J.C., et al. Annu. Rev. Immunol. 6, 251-281, (1988)

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4. Complement-fixing IgG1 constitutes a new subclass of mouse IgG Peter, L. EY, et al. Nature 281, 492 - 493, (1979)

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Sialic Acid on Herpes Simplex Virus Type 1 Envelope Glycoproteins Is Required for Efficient Infection of Cells Teuton, J.R., et al. J. Virol. 81, 3731-3739, (2007)

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SepBCTF4 Is Required for the Formation of DNA-Damage-Induced UvsCRAD51 Foci in Aspergillus nidulans Semighini, C.P., et al. Genetics 169, 1391-1402, (2005)

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The Protein Tyrosine Phosphatase-BL, Modulates Pancreatic β-cell Proliferation By Interaction With The Wnt Signalling Pathway Hannah, J., et al. J. Endocrinol. 197, 543 - 552, (2008)

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Msx1 and Msx2 are functional interacting partners of T-box factors in the regulation of Connexin43. Barnett, P., et al. Cardiovasc. Res. 78, 485-493, (2008)

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L-kynurenine Combined With Probenecid And The Novel Synthetic Kynurenic Acid Derivative Attenuate Nitroglycerin-induced NNOS In The Rat Caudal Trigeminal Nucleus. Vámos, E., et al. Neuropharmacology 57, 425-9, (2009)

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Bovine Intramuscular, Subcutaneous, And Perirenal Stromal-vascular Cells Express Similar Glucocorticoid Receptor Isoforms, But Exhibit Different Adipogenic Capacity. Ortiz-Colón, G., et al. J. Anim. Sci. 87, 1913-20, (2009)

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Multiple Peptides Homologous To Herpes Simplex Virus Type 1 Glycoprotein B Inhibit Viral Infection. Akkarawongsa, R., et al. Antimicrob. Agents Chemother. 53, 987-96, (2009)

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Production of recombinant anthrax toxin receptor (ATR/CMG2) fused with human Fc in planta. Andrianov, V., et al. Protein Expr. Purif. 70, 158-62, (2010)

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Possible role of TIEG1 as a feedback regulator of myostatin and TGF-beta in myoblasts. Miyake, M., et al. Biochem. Biophys. Res. Commun. 393, 762-6, (2010)

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A functional role for TorsinA in herpes simplex virus 1 nuclear egress. Maric, M., et al. J. Virol. 85, 9667-79, (2011)

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Influence of divalent metal ions on E2-induced ER pathway in goldfish (Carassius auratus) hepatocytes. Chang, Z., et al. Ecotox. Environ. Safety 74, 2233-9, (2011)

TIEG1 negatively controls the myoblast pool indispensable for fusion during myogenic differentiation of C2C12 cells. Miyake, M., et al. J. Cell Physiol. 226, 1128-36, (2011)

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SPINK3 modulates mouse sperm physiology through the reduction of nitric oxide level independently of its trypsin inhibitory activity. Zalazar, L., et al. Reproduction 143, 281-95, (2012)

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Expression and Localization of Lung Surfactant Protein A in Human Tissues Madsen, J. Am. J. Respir. Cell. Mol. Biol. 29, 591-597, (2003)

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