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  • A3563 - Anti-Mouse IgG (whole molecule) F(ab′)2 fragment–Alkaline Phosphatase antibody produced in sheep

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A3563 Sigma

Anti-Mouse IgG (whole molecule) F(ab′)2 fragment–Alkaline Phosphatase antibody produced in sheep

affinity isolated antibody, buffered aqueous glycerol solution

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Properties

Related Categories Alkaline Phosphatase, Alphabetical Index, Antibodies, MO-MR, Mouse Secondary Antibodies and Conjugates,
biological source   sheep
antibody form   affinity isolated antibody
clone   polyclonal
form   buffered aqueous glycerol solution
species reactivity   mouse
application(s)   direct ELISA: 1:30,000
  dot blot: 1:30,000
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
  western blot: 1:30,000
conjugate   alkaline phosphatase conjugate
shipped in   wet ice
storage temp.   2-8°C

Description

Immunogen

Mouse IgG

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol, and 15 mM sodium azide.

Application

Anti-Mouse IgG (whole molecule) F(ab’)2 fragment-Alkaline Phosphatase antibody is suitable for use in direct ELISA (1:30,000), western blot (1:30,000), and immunohistochemistry (at 1:50 dilution in formalin-fixed, paraffin-embedded sections). The antibody may also be used for dot blot and immunoblot assays using a working dilution of 1:30,000.

ELISAs using alkaline phosphatase conjugated sheep anti-mouse IgG whole molecule antibody were performed to measure the antibody response in the sera of mice immunixed to T. crassiceps. 1:1000 at 37 degrees for one hour and developed using p-nitrophenyl (Sigma).

General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice
Sheep anti-mouse IgG (whole molecule) F(ab’)2 fragment is specific for the F(ab’)2 of mouse IgG. The antibody preparation consists only of the F(ab’)2 fragment of sheep IgG as determined by SDS-PAGE. No contamination with sheep IgG whole molecule is observed.

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