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A3678 Sigma

Ammonium persulfate

for molecular biology, for electrophoresis, ≥98%

Synonym: AP, APS, Ammonium peroxodisulfate, Ammonium peroxydisulfate, PER

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Description

Analysis Note

Tested for use in acrylamide polymerization.

Application

Ammonium persulfate forms oxygen free radicals in aqueous solution by a base-catalyzed mechanism. The bases most commonly used as catalysts are tertiary amines such as N,N,N′,N′-tetramethylethylenediamine (TEMED) or 3-dimethylaminopropionitrile (DMAPN). The free radicals will cause acrylamide and bis-acrylamide to polymerize to form a gel matrix, which can be used for separating macromolecules by size.

Catalyst for acrylamide gel polymerization.

Packaging

25, 100 g in poly bottle

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
RIDADR 
UN 1444 5.1 / PGIII
WGK Germany 
1
RTECS 
SE0350000
Protocols & Articles

Articles

Introduction to PAGE

Polyacrylamide gels are formed by the reaction of acrylamide and bis-acrylamide (N,N’-methylenebisacrylamide) that results in highly cross-linked gel matrix. The gel acts as a sieve through which the...
Keywords: Buffers, Cell disruption, Electrophoresis, Gel electrophoresis, PAGE, Protein electrophoresis, Sample preparations, Separation, Western blot

NMR Analysis of Glycans

Nuclear magnetic resonance (NMR) spectroscopy measures the extent to which a glycan or other molecule distorts a magnetic field. This technique is non-destructive and can provide full structural info...
Glycobiology Analysis Manual, 2nd Edition
Keywords: Cancer, Digestions, Electrophoresis, Nuclear magnetic resonance spectroscopy, Separation, Spectroscopy

Peer-Reviewed Papers
15

References

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