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A3678 Sigma

Ammonium persulfate

for molecular biology, for electrophoresis, ≥98%

Synonym: AP, APS, Ammonium peroxodisulfate, Ammonium peroxydisulfate, PER

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Description

Analysis Note

Tested for use in acrylamide polymerization.

Application

Catalyst for acrylamide gel polymerization.

Ammonium persulfate has been used for the preparation of polyacrylamide gels and acrylamide hydrogels.

Packaging

25, 100 g in poly bottle

General description

Ammonium persulfate (APS) is a widely used reagent in biochemistry and molecular biology for the preparation of polyacrylamide gels. APS forms oxygen free radicals in aqueous solution by a base-catalyzed mechanism. The bases, most commonly used as catalysts, are tertiary amines such as TEMED (N,N,N′,N′-tetramethylethylenediamine) or DMAPN (3-dimethylaminopropionitrile). The free radicals will cause the polymerization of acrylamide and bis-acrylamide to form a gel matrix, which can be used for separating macromolecules by size. APS has also been utilized to study protein-protein interactions via photoinitiated crosslinking chemistry.

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
RIDADR 
UN 1444 5.1 / PGIII
WGK Germany 
1
RTECS 
SE0350000
Protocols & Articles

Articles

Introduction to PAGE

Polyacrylamide gels are formed by the reaction of acrylamide and bis-acrylamide (N,N’-methylenebisacrylamide) that results in highly cross-linked gel matrix. The gel acts as a sieve through which the...
Keywords: Buffers, Cell disruption, Electrophoresis, Gel electrophoresis, PAGE, Protein electrophoresis, Sample preparations, Separation, Western blot

NMR Analysis of Glycans

Nuclear magnetic resonance (NMR) spectroscopy measures the extent to which a glycan or other molecule distorts a magnetic field. This technique is non-destructive and can provide full structural info...
Glycobiology Analysis Manual, 2nd Edition
Keywords: Cancer, Digestions, Electrophoresis, Nuclear magnetic resonance spectroscopy, Separation, Spectroscopy

Peer-Reviewed Papers
15

References

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