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  • A3682 - Anti-Mouse IgG (Fab specific)–Peroxidase antibody produced in goat

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A3682 Sigma

Anti-Mouse IgG (Fab specific)–Peroxidase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibody Conjugates, FLAG Secondary Antibodies, FLAG System,
biological source   goat
antibody form   affinity isolated antibody
clone   polyclonal
form   buffered aqueous solution
species reactivity   mouse
does not react with   human, rat
application(s)   direct ELISA: 1:40,000
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150
  western blot: 1:80,000-160,000 using detecting β-actin in total cell extract of HeLa cells (5-10 μg per lane)
conjugate   peroxidase conjugate
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

purified mouse IgG, Fab fragment

Preparation Note

Adsorbed with human IgG and rat serum proteins to reduce background staining with human or rat samples.

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.01% thimerosal.

Application

Murine IgG levels were quantitated in brain abscess homogenates by ELISA using HRP conjugated goat anti-mouse Fab specific antibody as the detection antibody.

Goat anti-mouse IgG (Fab specific)-peroxidase antibody can be used for western blotting applications. The product can also be used for direct ELISA (1:40,000) and immunohistochemistry (at 1:150, using formalin-fixed, paraffin-embedded sections).

HRP conjugated goat anti-mouse IgG Fab specific was used as the secondary antibody for western blot analysis of bacterial samples of s. equi cells to detect fibrinogen-binding proteins.

General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.
Goat anti-mouse IgG (Fab specific)-peroxidase antibody is specific for mouse IgG and the Fab fragment of mouse IgG. The antibody reacts with all mouse IgG subclasses (G1, G2a, G2b and G3) and also with mouse IgA, IgM and IgE. Moreover, the antibody conjugate shows no reactivity with the Fc fragment of mouse IgG or with human and rat IgG.

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