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A3720 Sigma

Anti-ARTS antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-Apoptosis-Related Protein in the TGF-β Signaling Pathway

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Properties

Related Categories AR-AR, Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology,
species reactivity   human
application(s)   microarray: suitable
  western blot: 1:15,000 using a whole cell extract of HEK 293 cells transfected with ARTS expression plasmid
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 32 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... SEPT4(5414)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to the C-terminal region of human ARTS (amino acids 247-266 with N-terminal added lysine) conjugated to KLH.

General description

Apoptosis-related protein in TGF-β signaling pathway (ARTS) is a novel mitochondrial protein that mediates apoptosis induced by a variety of pro-apoptotic stimuli. The protein is derived form the human septin H5/PNUTL2/CSCrel-2b gene. Unlike other septins, ARTS is localized to mitochondria and translocates to the nucleus, coincident with apoptotic signals induced by TGF-β.
Rabbit anti-ARTS antibody specifically recognizes human ARTS (32kDa) by immunoblotting. Staining of ARTS in immunoblotting is specifically inhibited with the ARTS
immunizing peptide.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Immunoprecipitation of COS-7 cell lysates was performed using 10 μl of monoclonal mouse anti-ARTS antibody. Antibody was allowed to bind for 4 hours on ice before precipitated using G-sepharose beads.

Rabbit anti-ARTS antibody can be used for western blot at 1:15,000 using HEK 293 whole cell extracts transfected with ARTS expression plasmid. The antibody is also suitable for use in microarrays.

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