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A3812 Sigma

Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous glycerol solution

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Properties

Related Categories Alkaline Phosphatase, Alkaline Phosphatase Conjugates, Alphabetical Index, Antibodies, Antibody Conjugates,
biological source   goat
antibody form   affinity isolated antibody
clone   polyclonal
form   buffered aqueous glycerol solution
species reactivity   rabbit
application(s)   direct ELISA: 1:30,000
  dot blot: 1:30,000
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
  western blot: 1:30,000
conjugate   alkaline phosphatase conjugate
shipped in   wet ice
storage temp.   2-8°C

Description

Immunogen

Purified rabbit IgG

Preparation Note

Adsorbed to reduce background staining with human samples.

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 10 mM glycine, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide

Application

Citrullination of antithrombin by PADI4 was analyzed by ELISA using alkaline phosphatase conjugated goat anti-rabbit IgG as the secondary diluted at 1:5000 in 0.05M carbonate/bicarbonate buffer (Ph 9.6).

Goat anti-rabbit IgG (whole molecule)-alkaline phosphatase antibody can be used for western blot and ELISA. The product can laso be used for immunohistochemistry (1:50) and dot bot (1:30,000) assays.

General description

Anti-rabbit IgG (whole molecule) is produced in goat using purified rabbit IgG as the immunogen. Antibody is isolated from anti-rabbit IgG antiserum by immunospecific purification which removes essentially all goat serum proteins, including immunoglobulins that do not specifically bind to rabbit IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity in tissue or cell preparations. Anti-Rabbit IgG is conjugated to alkaline phosphatase by protein cross linking with 0.2% glutaraldehyde.
Specificity of the antiserum is determined by immunoelectrophoresis prior to conjugation, versus normal rabbit serum and rabbit IgG. Identity and purity of the antibody is established by immunoelectrophoresis prior to conjugation. Electrophoresis of the product followed by diffusion versus anti-goat IgG and anti-goat whole serum results in single arcs of precipitation.

IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins.

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How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
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